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| Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga | |
Ago, Hideo; Adachi, Hideyuki; Umena, Yasufumi2,3; Tashiro, Takayoshi4; Kawakami, Keisuke2; Kamiya, Nobuo2,4; Tian, Lirong; Han, Guangye; Kuang, Tingyun; Liu, Zheyi6; Wang, Fangjun6; Zou, Hanfa6; Enami, Isao7; Miyano, Masashi8; Shen, Jian-Ren
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| 2016 | |
| Source Publication | JOURNAL OF BIOLOGICAL CHEMISTRY
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| Volume | 291Issue:11Pages:5676-5687 |
| Abstract | Photosystem II (PSII) catalyzes light-induced water splitting, leading to the evolution of molecular oxygen indispensible for life on the earth. The crystal structure of PSII from cyanobacteria has been solved at an atomic level, but the structure of eukaryotic PSII has not been analyzed. Because eukaryotic PSII possesses additional subunits not found in cyanobacterial PSII, it is important to solve the structure of eukaryotic PSII to elucidate their detailed functions, as well as evolutionary relationships. Here we report the structure of PSII from a red alga Cyanidium caldarium at 2.76 resolution, which revealed the structure and interaction sites of PsbQ, a unique, fourth extrinsic protein required for stabilizing the oxygen-evolving complex in the lumenal surface of PSII. The PsbQ subunit was found to be located underneath CP43 in the vicinity of PsbV, and its structure is characterized by a bundle of four up-down helices arranged in a similar way to those of cyanobacterial and higher plant PsbQ, although helices I and II of PsbQ were kinked relative to its higher plant counterpart because of its interactions with CP43. Furthermore, two novel transmembrane helices were found in the red algal PSII that are not present in cyanobacterial PSII; one of these helices may correspond to PsbW found only in eukaryotic PSII. The present results represent the first crystal structure of PSII from eukaryotic oxygenic organisms, which were discussed in comparison with the structure of cyanobacterial PSII. |
| Keyword | crystal structure crystallography cyanobacteria electron transfer complex membrane protein photosynthesis photosystem II x-ray crystallography |
| Subject Area | Biochemistry & Molecular Biology |
| DOI | 10.1074/jbc.M115.711689 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | EXTRINSIC PROTEINS ; FUNCTIONAL-PROPERTIES ; CYANIDIUM-CALDARIUM ; CROSS-LINKING ; PSBQ PROTEIN ; ANGSTROM RESOLUTION ; 12-KDA PROTEIN ; CYANOBACTERIAL ; BINDING ; MECHANISM |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000372551800019 |
| Publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| Subtype | Article |
| Publication Place | BETHESDA |
| EISSN | 1083-351X |
| Funding Organization | JSPSMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [24000018] ; MEXT of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) ; Chinese Academy of SciencesChinese Academy of Sciences [KGZD-EW-T05] ; China State Key Basic Research Program Grant [2013CB911203] ; Creative Research Group Project by NSFC [21321064] |
| Corresponding Author Email | shen@cc.okayama-u.ac.jp |
| OA | Green Published, hybrid |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25321 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.RIKEN SPring 8 Ctr, Mikazuki, Hyogo 6795148, Japan 2.Okayama Univ, Fac Sci, Grad Sch Nat Sci & Technol, Photosynthesis Res Ctr, Okayama 7008530, Japan 3.Osaka City Univ, Adv Res Inst Nat Sci & Technol OCARNA, Sumiyoshi Ku, 3-3-138 Sugimoto, Osaka 7008530, Japan 4.Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PRESTO, Tokyo 1020076, Japan 5.Osaka City Univ, Grad Sch Sci, Dept Chem, Sumiyoshi Ku, Osaka 5588585, Japan 6.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 7.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 8.Tokyo Univ Sci, Fac Sci, Dept Biol, Shinjuku Ku, Tokyo 1628601, Japan 9.Aoyama Gakuin Univ, Dept Chem & Biol Sci, Sagamihara, Kanagawa 2525258, Japan |
| Recommended Citation GB/T 7714 | Ago, Hideo,Adachi, Hideyuki,Umena, Yasufumi,et al. Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(11):5676-5687. |
| APA | Ago, Hideo.,Adachi, Hideyuki.,Umena, Yasufumi.,Tashiro, Takayoshi.,Kawakami, Keisuke.,...&Shen, Jian-Ren.(2016).Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga.JOURNAL OF BIOLOGICAL CHEMISTRY,291(11),5676-5687. |
| MLA | Ago, Hideo,et al."Novel Features of Eukaryotic Photosystem II Revealed by Its Crystal Structure Analysis from a Red Alga".JOURNAL OF BIOLOGICAL CHEMISTRY 291.11(2016):5676-5687. |
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| Ago-2016-Novel Featu（5331KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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