Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening

doi:10.1007/s00425-015-2420-3

IB-CAS > 中科院光生物学重点实验室

	Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening
	Ning, De-Li ; Liu, Ke-Hui ; Liu, Chang-Cai ; Liu, Jin-Wen ; Qian, Chun-Rong ; Yu, Yang 9; Wang, Yue-Feng ; Wang, Ying-Chun ; Wang, Bai-Chen
	2016
发表期刊	PLANTA
ISSN	0032-0935
卷号	243 期号:2 页码:501-517
摘要	Main conclusion Large-scale comparative phosphoprotein analysis in maize seedlings reveals a complicated molecular regulation mechanism at the phosphoproteomic level during de-etiolation. In the present study we report a phosphoproteomic study conducted on Zea mays etiolated leaves harvested at three time points during greening (etiolated seedlings and seedlings exposed to light for 6 or 12 h). We identified a total of 2483 phosphopeptides containing 2389 unambiguous phosphosites from 1339 proteins. The abundance of nearly 692 phosphorylated peptides containing 783 phosphosites was reproducible and profiled with high confidence among treatments. Comparisons with other large-scale phosphoproteomic studies revealed that 473 of the phosphosites are novel to this study. Of the 783 phosphosites identified, 171, 79, and 138 were identified in 0, 6, and 12 h samples, respectively, which suggest that regulation of phosphorylation plays important roles during maize seedling de-etiolation. Our experimental methods included enrichment of phosphoproteins, allowing the identification of a great number of low abundance proteins, such as transcription factors, protein kinases, and photoreceptors. Most of the identified phosphoproteins were involved in gene transcription, post-transcriptional regulation, or signal transduction, and only a few were involved in photosynthesis and carbon metabolism. It is noteworthy that tyrosine phosphorylation and calcium signaling pathways might play important roles during maize seedling de-etiolation. Taken together, we have elucidated a new level of complexity in light-induced reversible protein phosphorylation during maize seedling de-etiolation.
关键词	De-etiolation Maize Phosphoprotein Proteomics Signal transduction
学科领域	Plant Sciences
DOI	10.1007/s00425-015-2420-3
收录类别	SCI
语种	英语
WOS关键词	ACTIVATED PROTEIN-KINASE ; NUCLEIC-ACID-BINDING ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; SIGNAL-TRANSDUCTION ; PHOSPHORYLATION ; ARABIDOPSIS ; LIGHT ; IDENTIFICATION ; GROWTH ; PHYTOCHROME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373310300017
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2048
资助机构	State Key Program of the National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31030017] ; Natural Sciences Foundation of Heilongjiang ProvinceNatural Science Foundation of Heilongjiang Province [C201010]
作者邮箱	ycwang@genetics.ac.cn ; wangbc@ibcas.ac.cn
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25325
专题	中科院光生物学重点实验室
作者单位	1.Qiqihar Med Univ, Inst Med, Qiqihar 161006, Peoples R China 2.[Ning, De-Li 3.Wang, Yue-Feng 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 5.[Liu, Ke-Hui 6.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 7.Res Inst Chem Def, State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China 8.Heilongjiang Bayi Agr Univ, Coll Life Sci & Biotechnol, Daqing 163319, Peoples R China 9.[Qian, Chun-Rong 10.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China
推荐引用方式 GB/T 7714	Ning, De-Li,Liu, Ke-Hui,Liu, Chang-Cai,et al. Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening[J]. PLANTA,2016,243(2):501-517.
APA	Ning, De-Li.,Liu, Ke-Hui.,Liu, Chang-Cai.,Liu, Jin-Wen.,Qian, Chun-Rong.,...&Wang, Bai-Chen.(2016).Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening.PLANTA,243(2),501-517.
MLA	Ning, De-Li,et al."Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening".PLANTA 243.2(2016):501-517.

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