The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein

doi:10.1038/srep19756

IB-CAS > 中科院光生物学重点实验室

	The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein
	Fang, Ying 1; Zhao, Shun 1; Zhang, Feilong 2; Zhao, Aiguo 3; Zhang, Wenxia ; Zhang, Min 2; Liu, Lin
	2016
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	Tetrapyrrole biosynthesis is an essential and tightly regulated process, and glutamyl-tRNA reductase (GluTR) is a key target for multiple regulatory factors at the post-translational level. By binding to the thylakoid membrane protein FLUORESCENT (FLU) or the soluble stromal GluTR-binding protein (GBP), the activity of GluTR is down-or up-regulated. Here, we reconstructed a ternary complex composed of the C-terminal tetratricopepetide-repeat domain of FLU, GBP, and GluTR, crystallized and solved the structure of the complex at 3.2 angstrom. The overall structure resembles the shape of merged two binary complexes as previously reported, and shows a large conformational change within GluTR. We also demonstrated that GluTR binds tightly with GBP but does not bind to GSAM under the same condition. These findings allow us to suggest a biological role of the ternary complex for the regulation of plant GluTR.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep19756
收录类别	SCI
语种	英语
WOS关键词	5-AMINOLEVULINIC ACID SYNTHESIS ; TETRAPYRROLE BIOSYNTHESIS ; CHLOROPHYLL BIOSYNTHESIS ; NEGATIVE REGULATOR ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; ENZYME ; EXPRESSION ; THALIANA ; HEMA1
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000368667700002
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370759, 31500243] ; National Basic Research Program of ChinaNational Basic Research Program of China [2011CBA00901] ; CASChinese Academy of Sciences [KGZD-EW-T05]
作者邮箱	liulin@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25326
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 4.Northwest A&F Univ, Coll Life Sci, Xian 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Fang, Ying,Zhao, Shun,Zhang, Feilong,et al. The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein[J]. SCIENTIFIC REPORTS,2016,6.
APA	Fang, Ying.,Zhao, Shun.,Zhang, Feilong.,Zhao, Aiguo.,Zhang, Wenxia.,...&Liu, Lin.(2016).The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein.SCIENTIFIC REPORTS,6.
MLA	Fang, Ying,et al."The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein".SCIENTIFIC REPORTS 6(2016).

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