Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis

doi:10.1111/jipb.12371

IB-CAS > 中科院光生物学重点实验室

	Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis
	Ding, Shunhua ; Wang, Liang 1; Yang, Zhipan ; Lu, Qingtao; Wen, Xiaogang ; Lu, Congming
	2016
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	58 期号:1 页码:29-47
摘要	Glutathione reductase (GR) catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH) and participates in the ascorbate-glutathione cycle, which scavenges H2O2. Here, we report that chloroplastic/mitochondrial GR2 is an important regulator of leaf senescence. Seed development of the homozygous gr2 knockout mutant was blocked at the globular stage. Therefore, to investigate the function of GR2 in leaf senescence, we generated transgenic Arabidopsis plants with decreased GR2 using RNAi. The GR2 RNAi plants displayed early onset of age-dependent and dark- and H2O2-induced leaf senescence, which was accompanied by the induction of the senescence-related marker genes SAG12 and SAG13. Furthermore, transcriptome analysis revealed that genes related to leaf senescence, oxidative stress, and phytohormone pathways were upregulated directly before senescence in RNAi plants. In addition, H2O2 accumulated to higher levels in RNAi plants than in wild-type plants and the levels of H2O2 peaked in RNAi plants directly before the early onset of leaf senescence. RNAi plants showed a greater decrease in GSH/GSSG levels than wild-type plants during leaf development. Our results suggest that GR2 plays an important role in leaf senescence by modulating H2O2 and glutathione signaling in Arabidopsis.
关键词	Arabidopsis thaliana ascorbate-glutathione cycle glutathione reductase2 hydrogen peroxide leaf senescence
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/jipb.12371
收录类别	SCI
语种	英语
WOS关键词	OXIDATIVE STRESS RESPONSES ; GENE-EXPRESSION ; HYDROGEN-PEROXIDE ; TRANSCRIPTION FACTOR ; SALICYLIC-ACID ; TRANSGENIC TOBACCO ; CELL-DEATH ; CHLOROPLASTS ; H2O2 ; BIOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000367731700003
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30970218] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105]
作者邮箱	lucm@ibcas.ac.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25328
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ding, Shunhua,Wang, Liang,Yang, Zhipan,et al. Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2016,58(1):29-47.
APA	Ding, Shunhua,Wang, Liang,Yang, Zhipan,Lu, Qingtao,Wen, Xiaogang,&Lu, Congming.(2016).Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,58(1),29-47.
MLA	Ding, Shunhua,et al."Decreased glutathione reductase2 leads to early leaf senescence in Arabidopsis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 58.1(2016):29-47.

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