Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids

doi:10.1007/s11120-015-0176-z

IB-CAS > 中科院光生物学重点实验室

	Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids
	Liu, Cheng ; Gao, Zhimin 1; Liu, Kun ; Sun, Ruixue ; Cui, Chunbo ; Holzwarth, Alfred R.; Yang, Chunhong
	2016
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	127 期号:1 页码:109-116
摘要	The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study, we simultaneously refolded and reconstituted functional pea PsbS into liposomes of thylakoid lipids starting from denatured expressed protein. Intrinsic fluorescence spectroscopy, trypsin digestion, and circular dichroism spectroscopy were used to characterize the native state of PsbS in the proteoliposomes. The functionality of refolded PsbS was further demonstrated by its effect on the fluorescence quenching of the major antenna system of photosystem II (LHCII) co-inserted into the liposomes. The fluorescence yield of native trimeric LHCII was lowered by PsbS by 50 % at neutral pH and by a further 25 % upon lowering the pH to 4.5. Furthermore, the acid-induced fluorescence reduction was completely reversed by addition of N,N'-dicyclohexylcarbodiimide, an inhibitor of protein protonation. These results indicate that reconstituted PsbS induces strong quenching of LHCII sensing changes in local pH via its protonation sites.
关键词	Photosynthesis PsbS Proteoliposomes Reconstitution Refolding
学科领域	Plant Sciences
DOI	10.1007/s11120-015-0176-z
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEX ; TIME-RESOLVED FLUORESCENCE ; PHOTOSYSTEM-II COMPLEXES ; PROTEIN CONTROLS ; IN-VITRO ; MEMBRANES ; SUBUNIT ; ANTENNA ; IDENTIFICATION ; ORGANIZATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000366156400013
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2011CBA00904] ; Key Research Program of the Chinese Academy of Sciences Grant [KSZD-EW-Z-018] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30800069, 31070212, 31370275, 31370588] ; European Union EU Training and Research Network Harvest ; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG HO-924/3-1]
作者邮箱	yangch@ibcas.ac.cn
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25329
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.State Forestry Adm, Int Ctr Bamboo & Rattan, Key Open Lab Bamboo & Rattan Sci & Technol, Beijing 100102, Peoples R China 3.Holzwarth, Alfred R.] Max Planck Inst Chem Energiekonvers, D-45470 Mulheim, Germany
推荐引用方式 GB/T 7714	Liu, Cheng,Gao, Zhimin,Liu, Kun,et al. Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids[J]. PHOTOSYNTHESIS RESEARCH,2016,127(1):109-116.
APA	Liu, Cheng.,Gao, Zhimin.,Liu, Kun.,Sun, Ruixue.,Cui, Chunbo.,...&Yang, Chunhong.(2016).Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids.PHOTOSYNTHESIS RESEARCH,127(1),109-116.
MLA	Liu, Cheng,et al."Simultaneous refolding of denatured PsbS and reconstitution with LHCII into liposomes of thylakoid lipids".PHOTOSYNTHESIS RESEARCH 127.1(2016):109-116.

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