Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube

doi:10.1093/jxb/erw160

	Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube
	Zhang, Meng 3; Zhang, Ruihui 3; Qu, Xiaolu 1,2; Huang, Shanjin 1,4
	2016
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	67 期号:11 页码:3407-3417
摘要	This study identifies FIM5 as an important player in constructing apical actin structure besides its defined role in constructing shank-oriented longitudinal actin cables in the pollen tube.The actin cytoskeleton is increasingly recognized as a major regulator of pollen tube growth. Actin filaments have distinct distribution patterns and dynamic properties within different regions of the pollen tube. Apical actin filaments are highly dynamic and crucial for pollen tube growth. However, how apical actin filaments are generated and properly constructed remains an open question. Here we showed that Arabidopsis fimbrin5 (FIM5) decorates filamentous structures throughout the entire tube but is apically concentrated. Apical actin structures are disorganized to different degrees in the pollen tubes of fim5 loss-of-function mutants. Further observations suggest that apical actin structures are not constructed properly because apical actin filaments cannot be maintained at the cortex of fim5 pollen tubes. Actin filaments appeared to be more curved in fim5 pollen tubes and this was confirmed by measurements showing that the convolutedness and the rate of change of convolutedness of actin filaments was significantly increased in fim5 pollen tubes. This suggests that the rigidity of the actin filaments may be compromised in fim5 pollen tubes. Further, the apical cell wall composition is altered, implying that tip-directed vesicle trafficking events are impaired in fim5 pollen tubes. Thus, we found that FIM5 decorates apical actin filaments and regulates their organization in order to drive polarized pollen tube growth.
关键词	Actin-binding protein actin bundles actin dynamics Arabidopsis thaliana fimbrin pollen tube
学科领域	Plant Sciences
DOI	10.1093/jxb/erw160
收录类别	SCI
语种	英语
WOS关键词	DEPOLYMERIZING FACTOR ; CELL-WALL ; POLARIZED GROWTH ; BINDING PROTEINS ; INTERACTING PROTEIN1 ; SPATIAL-DISTRIBUTION ; TIP GROWTH ; DYNAMICS ; TURNOVER ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000377471700021
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31125004, 31471266] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028]
作者邮箱	sjhuang@ibcas.ac.cn
作品OA属性	hybrid, Green Submitted, Green Published
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25334
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 3.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Meng,Zhang, Ruihui,Qu, Xiaolu,et al. Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(11):3407-3417.
APA	Zhang, Meng,Zhang, Ruihui,Qu, Xiaolu,&Huang, Shanjin.(2016).Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube.JOURNAL OF EXPERIMENTAL BOTANY,67(11),3407-3417.
MLA	Zhang, Meng,et al."Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube".JOURNAL OF EXPERIMENTAL BOTANY 67.11(2016):3407-3417.

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