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| Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube | |
| Zhang, Meng3; Zhang, Ruihui3; Qu, Xiaolu1,2; Huang, Shanjin1,4 | |
| 2016 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
 |
| ISSN | 0022-0957 |
| Volume | 67Issue:11Pages:3407-3417 |
| Abstract | This study identifies FIM5 as an important player in constructing apical actin structure besides its defined role in constructing shank-oriented longitudinal actin cables in the pollen tube.The actin cytoskeleton is increasingly recognized as a major regulator of pollen tube growth. Actin filaments have distinct distribution patterns and dynamic properties within different regions of the pollen tube. Apical actin filaments are highly dynamic and crucial for pollen tube growth. However, how apical actin filaments are generated and properly constructed remains an open question. Here we showed that Arabidopsis fimbrin5 (FIM5) decorates filamentous structures throughout the entire tube but is apically concentrated. Apical actin structures are disorganized to different degrees in the pollen tubes of fim5 loss-of-function mutants. Further observations suggest that apical actin structures are not constructed properly because apical actin filaments cannot be maintained at the cortex of fim5 pollen tubes. Actin filaments appeared to be more curved in fim5 pollen tubes and this was confirmed by measurements showing that the convolutedness and the rate of change of convolutedness of actin filaments was significantly increased in fim5 pollen tubes. This suggests that the rigidity of the actin filaments may be compromised in fim5 pollen tubes. Further, the apical cell wall composition is altered, implying that tip-directed vesicle trafficking events are impaired in fim5 pollen tubes. Thus, we found that FIM5 decorates apical actin filaments and regulates their organization in order to drive polarized pollen tube growth. |
| Keyword | Actin-binding protein actin bundles actin dynamics Arabidopsis thaliana fimbrin pollen tube |
| Subject Area | Plant Sciences |
| DOI | 10.1093/jxb/erw160 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | DEPOLYMERIZING FACTOR ; CELL-WALL ; POLARIZED GROWTH ; BINDING PROTEINS ; INTERACTING PROTEIN1 ; SPATIAL-DISTRIBUTION ; TIP GROWTH ; DYNAMICS ; TURNOVER ; PLANTS |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000377471700021 |
| Publisher | OXFORD UNIV PRESS |
| Subtype | Article |
| Publication Place | OXFORD |
| EISSN | 1460-2431 |
| Funding Organization | Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31125004, 31471266] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028] |
| Corresponding Author Email | sjhuang@ibcas.ac.cn |
| OA | hybrid, Green Submitted, Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25334 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 3.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Meng,Zhang, Ruihui,Qu, Xiaolu,et al. Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(11):3407-3417. |
| APA | Zhang, Meng,Zhang, Ruihui,Qu, Xiaolu,&Huang, Shanjin.(2016).Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube.JOURNAL OF EXPERIMENTAL BOTANY,67(11),3407-3417. |
| MLA | Zhang, Meng,et al."Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube".JOURNAL OF EXPERIMENTAL BOTANY 67.11(2016):3407-3417. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Zhang-2016-Arabidops（23143KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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