Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei

doi:10.1007/s00122-015-2627-5

	Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei
	Yeo, F. K. S.; Wang, Y.; Vozabova, T.; Huneau, C.; Leroy, P.; Chalhoub, B.; Qi, X. Q.; Niks, R. E.; Marcel, T. C.
	2016
发表期刊	THEORETICAL AND APPLIED GENETICS
ISSN	0040-5752
卷号	129 期号:2 页码:289-304
摘要	Key message Rphq2 , a minor gene for partial resistance to Puccinia hordei , was physically mapped in a 188 kbp introgression with suppressed recombination between haplotypes of rphq2 and Rphq2 barley cultivars. Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from cultivar Vada and line SusPtrit. Vada is immune to non-adapted Puccinia rust fungi, and partially resistant to P. hordei. SusPtrit is susceptible to several non-adapted rust fungi, and has been used for mapping QTLs for non-host and partial resistance. The BAC libraries help to identify genes determining the natural variation for partial and non-host resistances of barley to rust fungi. A major-effect QTL, Rphq2, for partial resistance to P. hordei was mapped in a complete Vada and an incomplete SusPtrit contig. The physical distance between the markers flanking Rphq2 was 195 Kbp in Vada and at least 226 Kbp in SusPtrit. This marker interval was predicted to contain 12 genes in either accession, of which only five genes were in common. The haplotypes represented by Vada and SusPtrit were found in 57 and 43 %, respectively, of a 194 barley accessions panel. The lack of homology between the two haplotypes probably explains the suppression of recombination in the Rphq2 area and limit further genetic resolution in fine mapping. The possible candidate genes for Rphq2 encode peroxidases, kinases and a member of seven-in-absentia protein family. This result suggests that Rphq2 does not belong to the NB-LRR gene family and does not resemble any of the partial resistance genes cloned previously.
学科领域	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1007/s00122-015-2627-5
收录类别	SCI
语种	英语
WOS关键词	ARTIFICIAL CHROMOSOME LIBRARY ; ISOLATE-SPECIFIC QTLS ; TRITICUM-AESTIVUM L. ; LEAF RUST ; DISEASE RESISTANCE ; BAC LIBRARY ; NONHOST RESISTANCE ; ISOGENIC LINES ; CONSENSUS MAP ; CONSTRUCTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000368991100007
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2242
资助机构	University Malaysia Sarawak, Malaysia
作者邮箱	rients.niks@wur.nl
作品OA属性	Green Published, hybrid, Green Accepted
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25335
专题	中科院植物分子生理学重点实验室
作者单位	1.[Yeo, F. K. S. 2.Vozabova, T. 3.Niks, R. E. 4.Marcel, T. C.] Wageningen Univ, Lab Plant Breeding, Droevendaalsesteeg 1,6708PB, NL-6700 AJ Wageningen, Netherlands 5.[Yeo, F. K. S.] Univ Malaysia Sarawak, Dept Plant Sci & Environm Ecol, Fac Resource Sci & Technol, Kota Samarahan 94300, Sarawak, Malaysia 6.[Wang, Y. 7.Qi, X. Q.] Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Nanxincun 20,Fragrant Hill, Beijing 100093, Peoples R China 8.[Vozabova, T.] Acad Sci Czech Republ, Inst Bot, Zamek 1, CS-25243 Pruhonice, Czech Republic 9.[Huneau, C.] INRA, UMR1165, Unite Rech Genom Vegetale, F-91057 Evry, France 10.[Huneau, C. 11.Chalhoub, B.] Univ Evry Val Essonne, UMR1165, Unite Rech Genom Vegetale, F-91057 Evry, France 12.[Leroy, P.] INRA, UMR1095, Genet Divers & Ecophysiol Cereals, F-63039 Clermont Ferrand, France 13.[Leroy, P.] Univ Blaise Pascal, UMR1095, Genet Divers & Ecophysiol Cereals, Clermont Ferrand, France 14.[Marcel, T. C.] INRA, UMR1290, BIOGER, F-78850 Thiverval Grignon, France 15.[Marcel, T. C.] AgroParisTech, UMR1290, BIOGER, F-78850 Thiverval Grignon, France
推荐引用方式 GB/T 7714	Yeo, F. K. S.,Wang, Y.,Vozabova, T.,et al. Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei[J]. THEORETICAL AND APPLIED GENETICS,2016,129(2):289-304.
APA	Yeo, F. K. S..,Wang, Y..,Vozabova, T..,Huneau, C..,Leroy, P..,...&Marcel, T. C..(2016).Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei.THEORETICAL AND APPLIED GENETICS,129(2),289-304.
MLA	Yeo, F. K. S.,et al."Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei".THEORETICAL AND APPLIED GENETICS 129.2(2016):289-304.

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