Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice

doi:10.1371/journal.pgen.1006085

	Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice
	Liu, Lingtong 1; Zheng, Canhui 1; Kuang, Baijan ; Wei, Liqin ; Yan, Longfeng 2; Wang, Tai
	2016
发表期刊	PLOS GENETICS
ISSN	1553-7404
卷号	12 期号:7
摘要	During sexual reproduction of flowering plants, the pollen tube grows fast and over a long distance within the pistil to deliver two sperms for double fertilization. Growing plant cells need to communicate constantly with external stimuli as well as monitor changes in surface tension of the cell wall and plasma membrane to coordinate these signals and internal growth machinery; however, the underlying mechanisms remain largely unknown. Here we show that the rice member of plant-specific receptor-like kinase CrRLK1Ls subfamily, Ruptured Pollen tube (RUPO), is specifically expressed in rice pollen. RUPO localizes to the apical plasma membrane and vesicle of pollen tubes and is required for male gamete transmission. K+ levels were greater in pollen of homozygous CRISPR-knockout lines than wildtype plants, and pollen tubes burst shortly after germination. We reveal the interaction of RUPO with high-affinity potassium transporters. Phosphorylation of RUPO established and dephosphorylation abolished the interaction. These results have revealed the receptor-like kinase as a regulator of high-affinity potassium transporters via phosphorylation-dependent interaction, and demonstrated a novel receptor-like kinase signaling pathway that mediates K+ homeostasis required for pollen tube growth and integrity.
学科领域	Genetics & Heredity
DOI	10.1371/journal.pgen.1006085
收录类别	SCI
语种	英语
WOS关键词	DNA INSERTIONAL MUTAGENESIS ; SACCHAROMYCES-CEREVISIAE ; ARABIDOPSIS-THALIANA ; ORYZA-SATIVA ; CELL-GROWTH ; PLANT-CELL ; CATHARANTHUS-ROSEUS ; SIGNALING NETWORKS ; ANTHER DEHISCENCE ; K+ TRANSPORTERS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000381050100003
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CB945101, 2012CB910504] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31121065]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Green Submitted, gold, Green Published
引用统计	被引频次：89[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25337
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Lanzhou Univ, Coll Life Sci, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lingtong,Zheng, Canhui,Kuang, Baijan,et al. Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice[J]. PLOS GENETICS,2016,12(7).
APA	Liu, Lingtong,Zheng, Canhui,Kuang, Baijan,Wei, Liqin,Yan, Longfeng,&Wang, Tai.(2016).Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice.PLOS GENETICS,12(7).
MLA	Liu, Lingtong,et al."Receptor-Like Kinase RUPO Interacts with Potassium Transporters to Regulate Pollen Tube Growth and Integrity in Rice".PLOS GENETICS 12.7(2016).

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