The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1

doi:10.1111/tpj.13158

	The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1
	Guo, Lei 1; Jiang, Li 4; Zhang, Ying ; Lu, Xiu-Li ; Xie, Qi 2; Weijers, Dolf 3; Liu, Chun-Ming
	2016
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	86 期号:2 页码:161-174
摘要	As the start of a new life cycle, activation of the first division of the zygote is a critical event in both plants and animals. Because the zygote in plants is difficult to access, our understanding of how this process is achieved remains poor. Here we report genetic and cell biological analyses of the zygote-arrest 1 (zyg1) mutant in Arabidopsis, which showed zygote-lethal and over-accumulation of cyclin B1 D-box-GUS in ovules. Map-based cloning showed that ZYG1 encodes the anaphase-promoting complex/cyclosome (APC/C) subunit 11 (APC11). Live-cell imaging studies showed that APC11 is expressed in both egg and sperm cells, in zygotes and during early embryogenesis. Using a GFP-APC11 fusion construct that fully complements zyg1, we showed that GFP-APC11 expression persisted throughout the mitotic cell cycle, and localized to cell plates during cytokinesis. Expression of non-degradable cyclin B1 in the zygote, or mutations of either APC1 or APC4, also led to a zyg1-like phenotype. Biochemical studies showed that APC11 has self-ubiquitination activity and is able to ubiquitinate cyclin B1 and promote degradation of cyclin B1. These results together suggest that APC/C-mediated degradation of cyclin B1 in Arabidopsis is critical for initiating the first division of the zygote.
关键词	APC11 cyclosome zygote activation cell cycle ubiquitination Arabidopsis thaliana
学科领域	Plant Sciences
DOI	10.1111/tpj.13158
收录类别	SCI
语种	英语
WOS关键词	FEMALE GAMETOGENESIS ; SEED DEVELOPMENT ; CELL-CYCLE ; PROTEIN ; ENCODES ; EMBRYO ; APC/C ; COMPLEX/CYCLOSOME ; EMBRYOGENESIS ; TRANSITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000375103400004
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943401, 2013CB967301] ; Sino-Dutch International Collaboration Project [31161130531]
作者邮箱	cmliu@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25339
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 4.Wageningen Univ, Biochem Lab, Dreijenlaan 3, NL-6703 HA Wageningen, Netherlands 5.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China
推荐引用方式 GB/T 7714	Guo, Lei,Jiang, Li,Zhang, Ying,et al. The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1[J]. PLANT JOURNAL,2016,86(2):161-174.
APA	Guo, Lei.,Jiang, Li.,Zhang, Ying.,Lu, Xiu-Li.,Xie, Qi.,...&Liu, Chun-Ming.(2016).The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1.PLANT JOURNAL,86(2),161-174.
MLA	Guo, Lei,et al."The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1".PLANT JOURNAL 86.2(2016):161-174.

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