Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean

doi:10.1104/pp.16.01132

	Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean
	Li, Shuo ; Wang, Nan ; Ji, Dandan 1; Xue, Zheyong 2; Yu, Yanchong ; Jiang, Yupei ; Liu, Jinglin ; Liu, Zhenhua ; Xiang, Fengning
	2016
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	172 期号:3 页码:1804-1820
摘要	Functional divergence is thought to be an important evolutionary driving force for the retention of duplicate genes. We reconstructed the evolutionary history of soybean (Glycine max) membrane-bound NAC transcription factor (NTL) genes. NTLs are thought to be components of stress signaling and unique in their requirement for proteolytic cleavage to free them from the membrane. Most of the 15 GmNTL genes appear to have evolved under strong purifying selection. By analyzing the phylogenetic tree and gene synteny, we identified seven duplicate gene pairs generated by the latest whole-genome duplication. The members of each pair were shown to have variously diverged at the transcriptional (organ specificity and responsiveness to stress), posttranscriptional (alternative splicing), and protein (proteolysis-mediated membrane release and transactivation activity) levels. The dormant (full-length protein) and active (protein without a transmembrane motif) forms of one pair of duplicated gene products (GmNTL1/GmNLT11) were each separately constitutively expressed in Arabidopsis (Arabidopsis thaliana). The heteroexpression of active but not dormant forms of these proteins caused improved tolerance to abiotic stresses, suggesting that membrane release was required for their functionality. Arabidopsis carrying the dormant form of GmNTL1 was more tolerant to hydrogen peroxide, which induces its membrane release. Tolerance was not increased in the line carrying dormant GmNTL11, which was not released by hydrogen peroxide treatment. Thus, NTL-release pattern changes may cause phenotypic divergence. It was concluded that a variety of functional divergences contributed to the retention of these GmNTL duplicates.
学科领域	Plant Sciences
DOI	10.1104/pp.16.01132
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; DUPLICATED GENES ; SUBCELLULAR RELOCALIZATION ; GENOME DUPLICATIONS ; POSITIVE SELECTION ; LEAF SENESCENCE ; STRESS-RESPONSE ; MESSENGER-RNA ; DIVERGENCE ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000391172300034
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National High-Technology Research and Development Program 863 of ChinaNational High Technology Research and Development Program of China [2013AA102602-4] ; National Key Research and Development Program of China [2016YFD0101902] ; National Research and Development Project of Transgenic Crops of the Ministry of Science and Technology of China [2013ZX08010002-002] ; Key Projects in the National Science and Technology Pillar Program in the 12th Five-Year Plan of China [2011BAI06B01] ; Chinese National Special Science Research Program [2013CB967303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31201269, 30970243, 31270328] ; International Technology Cooperation Project of Shandong Province of China [2011176] ; Chinese Natural Education Ministry Doctor Station Foundation Fellowship [913111006, 20120131120028] ; Science and Technology Plan of Shandong Province [2013GNC11010]
作者邮箱	xfn0990@sdu.edu.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25340
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Shandong, Peoples R China 2.Qilu Univ Technol, Jinan 250353, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Fragrant Hill, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Shuo,Wang, Nan,Ji, Dandan,et al. Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean[J]. PLANT PHYSIOLOGY,2016,172(3):1804-1820.
APA	Li, Shuo.,Wang, Nan.,Ji, Dandan.,Xue, Zheyong.,Yu, Yanchong.,...&Xiang, Fengning.(2016).Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean.PLANT PHYSIOLOGY,172(3),1804-1820.
MLA	Li, Shuo,et al."Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean".PLANT PHYSIOLOGY 172.3(2016):1804-1820.

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