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An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea | |
Liu, Yanling1; Zhao, Zhongjuan2; Xue, Zheyong3; Wang, Long; Cai, Yunfei; Wang, Peng; Wei, Tiandi; Gong, Jing; Liu, Zhenhua; Li, Juan; Li, Shuo; Xiang, Fengning | |
2016 | |
发表期刊 | SCIENTIFIC REPORTS |
ISSN | 2045-2322 |
卷号 | 6 |
摘要 | Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of the Gentiana straminea gene GsAS2 that encodes a beta-amyrin synthase (beta AS) enzyme. Unlike its previously isolated paralog GsAS1, GsAS2 lacks introns. Its predicted protein product was is a 759 residue polypeptide that shares high homology with other known beta-amyrin synthases (beta ASs). Heterologously expressed GsAS2 generates more beta-amyrin in yeast than does GsAS1. Constitutive over-expression of GsAS2 resulted in a 5.7 fold increase in oleanolic acid accumulation, while over-expression of GsAS1 led to a 3 fold increase. Additionally, RNAi-directed suppression of GsAS2 and GsAS1 in G. straminea decreased oleonolic acid levels by 65.9% and 21% respectively, indicating that GsAS2 plays a more important role than GsAS1 in oleanolic acid biosynthesis in G. straminea. We uses a docking model to explore the catalytic mechanism of GsAS1/2 and predicted that GsAS2, with its Y560, have higher efficiency than GsAS1 and mutated versions of GsAS2 in beta-amyrin produce. When the key residue in GsAS2 was mutagenized, it produced about 41.29% and 71.15% less beta-amyrin than native, while the key residue in GsAS1 was mutagenized to that in GsAS2, the mutant produced 38.02% more beta-amyrin than native GsAS1. |
学科领域 | Multidisciplinary Sciences |
DOI | 10.1038/srep33364 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | EUPHORBIA-TIRUCALLI L ; MULTIFUNCTIONAL TRITERPENE SYNTHASE ; OXIDOSQUALENE CYCLASES ; ARABIDOPSIS-THALIANA ; FUNCTIONAL-ANALYSIS ; MAXIMUM-LIKELIHOOD ; SQUALENE SYNTHASE ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; PLANTS |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000383575000001 |
出版者 | NATURE PUBLISHING GROUP |
文献子类 | Article |
出版地 | LONDON |
资助机构 | National Key Research and Development Program of China [2016YFD0101902] ; National High Technology Research and Development Program 863National High Technology Research and Development Program of China [2013AA102602-4] ; National Special Science Research Program of China [2013CB967303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270328, 31471515, 31200226, 31500232, 31370337] ; National Transgenic Project of China [2013ZX08010002-002] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M551893] ; Science & Technology Plan of Shandong Province [2013GNC11010] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2015PC009] ; Research Program for International S&T Cooperation Projects of Shandong Province [2011176] |
作者邮箱 | xfn0990@sdu.edu.cn |
作品OA属性 | Green Published, gold |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25343 |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Peoples R China 2.Laiwu Vocat & Tech Coll, Dept Informat Engn, Laiwu 271100, Peoples R China 3.Shandong Acad Sci, Inst Ecol, Key Lab Appl Microbiol Shandong Prov, Jinan 250014, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Nanxincun 20,Fragrant Hill, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Yanling,Zhao, Zhongjuan,Xue, Zheyong,et al. An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Liu, Yanling.,Zhao, Zhongjuan.,Xue, Zheyong.,Wang, Long.,Cai, Yunfei.,...&Xiang, Fengning.(2016).An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.SCIENTIFIC REPORTS,6. |
MLA | Liu, Yanling,et al."An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea".SCIENTIFIC REPORTS 6(2016). |
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