IB-CAS  > 中科院植物分子生理学重点实验室
An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea
Liu, Yanling1; Zhao, Zhongjuan2; Xue, Zheyong3; Wang, Long; Cai, Yunfei; Wang, Peng; Wei, Tiandi; Gong, Jing; Liu, Zhenhua; Li, Juan; Li, Shuo; Xiang, Fengning
2016
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号6
摘要Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of the Gentiana straminea gene GsAS2 that encodes a beta-amyrin synthase (beta AS) enzyme. Unlike its previously isolated paralog GsAS1, GsAS2 lacks introns. Its predicted protein product was is a 759 residue polypeptide that shares high homology with other known beta-amyrin synthases (beta ASs). Heterologously expressed GsAS2 generates more beta-amyrin in yeast than does GsAS1. Constitutive over-expression of GsAS2 resulted in a 5.7 fold increase in oleanolic acid accumulation, while over-expression of GsAS1 led to a 3 fold increase. Additionally, RNAi-directed suppression of GsAS2 and GsAS1 in G. straminea decreased oleonolic acid levels by 65.9% and 21% respectively, indicating that GsAS2 plays a more important role than GsAS1 in oleanolic acid biosynthesis in G. straminea. We uses a docking model to explore the catalytic mechanism of GsAS1/2 and predicted that GsAS2, with its Y560, have higher efficiency than GsAS1 and mutated versions of GsAS2 in beta-amyrin produce. When the key residue in GsAS2 was mutagenized, it produced about 41.29% and 71.15% less beta-amyrin than native, while the key residue in GsAS1 was mutagenized to that in GsAS2, the mutant produced 38.02% more beta-amyrin than native GsAS1.
学科领域Multidisciplinary Sciences
DOI10.1038/srep33364
收录类别SCI
语种英语
WOS关键词EUPHORBIA-TIRUCALLI L ; MULTIFUNCTIONAL TRITERPENE SYNTHASE ; OXIDOSQUALENE CYCLASES ; ARABIDOPSIS-THALIANA ; FUNCTIONAL-ANALYSIS ; MAXIMUM-LIKELIHOOD ; SQUALENE SYNTHASE ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; PLANTS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000383575000001
出版者NATURE PUBLISHING GROUP
文献子类Article
出版地LONDON
资助机构National Key Research and Development Program of China [2016YFD0101902] ; National High Technology Research and Development Program 863National High Technology Research and Development Program of China [2013AA102602-4] ; National Special Science Research Program of China [2013CB967303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270328, 31471515, 31200226, 31500232, 31370337] ; National Transgenic Project of China [2013ZX08010002-002] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M551893] ; Science & Technology Plan of Shandong Province [2013GNC11010] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2015PC009] ; Research Program for International S&T Cooperation Projects of Shandong Province [2011176]
作者邮箱xfn0990@sdu.edu.cn
作品OA属性Green Published, gold
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/25343
专题中科院植物分子生理学重点实验室
作者单位1.Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Peoples R China
2.Laiwu Vocat & Tech Coll, Dept Informat Engn, Laiwu 271100, Peoples R China
3.Shandong Acad Sci, Inst Ecol, Key Lab Appl Microbiol Shandong Prov, Jinan 250014, Peoples R China
4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Nanxincun 20,Fragrant Hill, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yanling,Zhao, Zhongjuan,Xue, Zheyong,et al. An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea[J]. SCIENTIFIC REPORTS,2016,6.
APA Liu, Yanling.,Zhao, Zhongjuan.,Xue, Zheyong.,Wang, Long.,Cai, Yunfei.,...&Xiang, Fengning.(2016).An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.SCIENTIFIC REPORTS,6.
MLA Liu, Yanling,et al."An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea".SCIENTIFIC REPORTS 6(2016).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Liu-2016-An Intronle(4301KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Yanling]的文章
[Zhao, Zhongjuan]的文章
[Xue, Zheyong]的文章
百度学术
百度学术中相似的文章
[Liu, Yanling]的文章
[Zhao, Zhongjuan]的文章
[Xue, Zheyong]的文章
必应学术
必应学术中相似的文章
[Liu, Yanling]的文章
[Zhao, Zhongjuan]的文章
[Xue, Zheyong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Liu-2016-An Intronless beta-amyrin Synthase Ge.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。