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| The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins | |
| Zhang, Ruihui3; Chang, Ming1; Zhang, Meng3; Wu, Youjun4; Qu, Xiaolu1,2; Huang, Shanjin1 | |
| 2016 | |
| 发表期刊 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 卷号 | 291期号:34页码:17881-17896 |
| 摘要 | Fimbrins/plastins have been implicated in the generation of distinct actin structures, which are linked to different cellular processes. Historically, fimbrins/plastins were mainly considered as generating tight actin bundles. Here, we demonstrate that different members of the fimbrin/plastin family have diverged biochemically during evolution to generate either tight actin bundles or loose networks with distinct biochemical and biophysical properties. Using the phylogenetically and functionally distinct Arabidopsis fimbrins FIM4 and FIM5 we found that FIM4 generates both actin bundles and cross-linked actin filaments, whereas FIM5 only generates actin bundles. The distinct functions of FIM4 and FIM5 are clearly observed at single-filament resolution. Domain swapping experiments showed that cooperation between the conformationally plastic calponin-homology domain 2 (CH2) and the N-terminal headpiece determines the function of the full-length protein. Our study suggests that the structural plasticity of fimbrins/plastins has biologically meaningful consequences, and provides novel insights into the structure-function relationship of fimbrins/plastins as well as shedding light on how cells generate distinct actin structures. |
| 关键词 | actin Arabidopsis Arp2 3 complex cytoskeleton microfilaments plant |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1074/jbc.M116.730069 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | POLLEN-TUBE GROWTH ; ACTIN-BINDING-PROTEINS ; CALPONIN HOMOLOGY DOMAINS ; ALPHA-ACTININ ; F-ACTIN ; ARP2/3 COMPLEX ; MUSCULAR-DYSTROPHY ; MISSENSE MUTATIONS ; FISSION YEAST ; HUMAN FIMBRIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000383241300032 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 文献子类 | Article |
| 出版地 | BETHESDA |
| EISSN | 1083-351X |
| 资助机构 | Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31125004] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028] |
| 作者邮箱 | sjhuang@tsinghua.edu.cn |
| 作品OA属性 | Green Published, hybrid |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25348 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 3.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Ruihui,Chang, Ming,Zhang, Meng,et al. The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(34):17881-17896. |
| APA | Zhang, Ruihui,Chang, Ming,Zhang, Meng,Wu, Youjun,Qu, Xiaolu,&Huang, Shanjin.(2016).The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins.JOURNAL OF BIOLOGICAL CHEMISTRY,291(34),17881-17896. |
| MLA | Zhang, Ruihui,et al."The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins".JOURNAL OF BIOLOGICAL CHEMISTRY 291.34(2016):17881-17896. |
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| Zhang-2016-The Struc（5248KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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