Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana

doi:10.1111/tpj.13211

	Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana
	Liu, Zhenhua ; Li, Juan ; Wang, Long ; Li, Qiang ; Lu, Qing ; Yu, Yanchong ; Li, Shuo ; Bai, Ming-Yi ; Hu, Yuxin1 ; Xiang, Fengning
	2016
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	87 期号:4 页码:391-402
摘要	In tissue culture systems plant cells can be induced to regenerate to whole plants. A particularly striking example of cellular reprogramming is seen in this regeneration process, which typically begins with the induction of an intermediate cell mass referred to as callus. The identity of the key genetic cues associated with callus formation is still largely unknown. Here a microRNA-directed phytohormonal interaction is described which represses callus initiation and formation in Arabidopsis thaliana. miR160 and ARF10 (At2g28350), a gene encoding an auxin response factor, were shown to exhibit a contrasting pattern of transcription during callus initiation from pericycle-like cells. The callus initiation is faster and more prolific in a miR160-resistant form of ARF10 (mARF10), but slower and less prolific in the transgenic line over-expressing miR160c (At5g46845), arf10 and arf10 arf16 mutants than that in the wild type. ARF10 repressed the expression of Arabidopsis Response Regulator15 (ARR15, At1g74890) via its direct binding to the gene's promoter. The loss of function of ARR15 enhanced callus initiation and partly rescued the phenotype induced by the transgene Pro35S:miR160c. Overexpression of ARR15 partly rescues the callus initiation defect of mARF10 plants. Our findings define miR160 as a key repressor of callus formation and reveal that the initiation of callus is repressed by miR160-directed interaction between auxin and cytokinin.
关键词	miR160 ARF10 callus auxin-cytokinin interaction auxin cytokinin Arabidopsis thaliana
学科领域	Plant Sciences
DOI	10.1111/tpj.13211
收录类别	SCI
语种	英语
WOS关键词	LATERAL ROOT ORGANOGENESIS ; RESPONSE-FACTOR ; HORMONAL-CONTROL ; SHOOT MERISTEM ; IN-VITRO ; PLANTS ; REGENERATION ; EXPRESSION ; TRANSCRIPTION ; GENES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000383712200005
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Special Science Research Program of China [2013CB967300] ; National Key Research and Development Program of China [2016YFD0101902] ; National High Technology Research and Development Program '863'National High Technology Research and Development Program of China [2013AA102602-4] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31500232, 31270328, 30970243, 31471515] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2014CP002] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M572013] ; Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province [BS2013SW027] ; National Transgenic Project of China [2013ZX08010002-002] ; Science and Technology Plan of Shandong Province [2013GNC11010] ; Research Program for International S&T Cooperation Projects of Shandong Province [2011176]
作者邮箱	xfn0990@sdu.edu.cn
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25349
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhenhua,Li, Juan,Wang, Long,et al. Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana[J]. PLANT JOURNAL,2016,87(4):391-402.
APA	Liu, Zhenhua.,Li, Juan.,Wang, Long.,Li, Qiang.,Lu, Qing.,...&Xiang, Fengning.(2016).Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana.PLANT JOURNAL,87(4),391-402.
MLA	Liu, Zhenhua,et al."Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana".PLANT JOURNAL 87.4(2016):391-402.

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