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CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor | |
Zhao, Shuangshuang1; Jiang, Yuxiang2; Zhao, Yang1; Huang, Shanjin2,3; Yuan, Ming1; Zhao, Yanxiu; Guo, Yan1 | |
2016 | |
发表期刊 | PLANT CELL |
ISSN | 1040-4651 |
卷号 | 28期号:6页码:1422-1439 |
摘要 | The opening and closing of stomata are crucial for plant photosynthesis and transpiration. Actin filaments undergo dynamic reorganization during stomatal closure, but the underlying mechanism for this cytoskeletal reorganization remains largely unclear. In this study, we identified and characterized Arabidopsis thaliana casein kinase 1-like protein 2 (CKL2), which responds to abscisic acid (ABA) treatment and participates in ABA- and drought-induced stomatal closure. Although CKL2 does not bind to actin filaments directly and has no effect on actin assembly in vitro, it colocalizes with and stabilizes actin filaments in guard cells. Further investigation revealed that CKL2 physically interacts with and phosphorylates actin depolymerizing factor 4 (ADF4) and inhibits its activity in actin filament disassembly. During ABA-induced stomatal closure, deletion of CKL2 in Arabidopsis alters actin reorganization in stomata and renders stomatal closure less sensitive to ABA, whereas deletion of ADF4 impairs the disassembly of actin filaments and causes stomatal closure to be more sensitive to ABA. Deletion of ADF4 in the ckl2 mutant partially recues its ABA-insensitive stomatal closure phenotype. Moreover, Arabidopsis ADFs from subclass I are targets of CKL2 in vitro. Thus, our results suggest that CKL2 regulates actin filament reorganization and stomatal closure mainly through phosphorylation of ADF. |
学科领域 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
DOI | 10.1105/tpc.16.00078 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | POLLEN-TUBE GROWTH ; ABSCISIC-ACID ; GUARD-CELLS ; HYDROGEN-PEROXIDE ; SIGNAL-TRANSDUCTION ; COFILIN PHOSPHORYLATION ; INTERACTING PROTEIN-1 ; BINDING PROTEIN ; CORTICAL ARRAY ; NITRIC-OXIDE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000380689400018 |
出版者 | AMER SOC PLANT BIOLOGISTS |
文献子类 | Article |
出版地 | ROCKVILLE |
EISSN | 1532-298X |
资助机构 | National Basic Research Program of ChinaNational Basic Research Program of China [2015CB910202] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31430012] ; Foundation for Innovative Research Group of the National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31121002] |
作者邮箱 | zhaoyx@sdnu.edu.cn ; guoyan@cau.edu.cn |
作品OA属性 | Green Published, Bronze |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25352 |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Shandong Normal Univ, Life Sci Coll, Key Lab Plant Stress, Jinan 250014, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Shuangshuang,Jiang, Yuxiang,Zhao, Yang,et al. CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor[J]. PLANT CELL,2016,28(6):1422-1439. |
APA | Zhao, Shuangshuang.,Jiang, Yuxiang.,Zhao, Yang.,Huang, Shanjin.,Yuan, Ming.,...&Guo, Yan.(2016).CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor.PLANT CELL,28(6),1422-1439. |
MLA | Zhao, Shuangshuang,et al."CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor".PLANT CELL 28.6(2016):1422-1439. |
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