Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms

doi:10.3389/fpls.2016.00707

	Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms
	Yu, Huatao 1; Wang, Tai
	2016
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	7
摘要	Starch biosynthesis and starch granule packaging in cereal endosperms involve a coordinated action of starch biosynthesis enzymes and coordination with other metabolisms. Because directly binding to starch granules, starch granule-associated proteins (SGAPs) are essential to understand the underlying mechanisms, however the information on SGAPs remains largely unknown. Here, we dissected developmentally changed SGAPs from developing rice endosperms from 10 to 20 days after flowering (DAF). Starch granule packaging was not completed at 10 DAF, and was finished in the central endosperm at 15 DAF and in the whole endosperm at 20 DAF. Proteomic analysis with two-dimensional differential in-gel electrophoresis and mass spectrometry revealed 115 developmentally changed SGAPs, representing 37 unique proteins. 65% of the unique proteins had isoforms. 39% of the identified SGAPs were involved in starch biosynthesis with main functions in polyglucan elongation and granule structure trimming. Almost all proteins involved in starch biosynthesis, amino acid biosynthesis, glycolysis, protein folding, and PPDK pathways increased abundance as the endosperm developed, and were predicted in an interaction network. The network represents an important mechanism to orchestrate carbon partitioning among starch biosynthesis, amino acid biosynthesis and glycolysis for efficient starch and protein storage. These results provide novel insights into mechanisms of starch biosynthesis and its coordination with amino acid metabolisms and glycolysis in cereal endosperms.
关键词	starch granules starch granules-associated proteins endosperm starch biosynthesis rice
学科领域	Plant Sciences
DOI	10.3389/fpls.2016.00707
收录类别	SCI
语种	英语
WOS关键词	TRITICUM-AESTIVUM L. ; WHEAT ; AMYLOPLASTS ; BARLEY ; MAIZE ; ISOAMYLASE ; ENZYMES ; EXTRACTION ; INITIATION ; COMPLEXES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000376745000001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CBA01402, 2012CB910504]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25353
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yu, Huatao,Wang, Tai. Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Yu, Huatao,&Wang, Tai.(2016).Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms.FRONTIERS IN PLANT SCIENCE,7.
MLA	Yu, Huatao,et al."Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms".FRONTIERS IN PLANT SCIENCE 7(2016).

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