The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis

doi:10.3389/fpls.2016.00532

	The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis
	Li, Qi 1; Deng, Zhuyun ; Gong, Chunyan ; Wang, Tai
	2016
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	7
摘要	Microgametogenesis is the post-meiotic pollen developmental phase when unicellular microspores develop into mature tricellular pollen. In rice, microgametogenesis can influence grain yields to a great degree because pollen abortion occurs more easily during microgametogenesis than during other stages of pollen development. However, our knowledge of the genes involved in microgametogenesis in rice remains limited. Due to the dependence of pollen development on the regulatory mechanisms of protein expression, we identified the encoding gene of the eukaryotic translation initiation factor 3, subunit fin Oryza saliva (OseIF3f). Immunoprecipitation combined with mass spectrometry confirmed that OseIF3f was a subunit of rice elF3, which consisted of at least 12 subunits including elF3a, eIF3b, elF3c, elF3d, elF3e, elF3f, elF3g, elF3h, elF3i, elF3k, elF3l, and elF3m. OseIF3f showed high mRNA levels in immature florets and is highly abundant in developing anthers. Subcellular localization analysis showed that OseIF3f was localized to the cytosol and the endoplasmic reticulum in rice root cells. We further analyzed the biological function of OseIF3f using the double-stranded RNA-mediated interference (RNAi) approach. The OseIF3f-RNAi lines grew normally at the vegetative stage but displayed a large reduction in seed production and pollen viability, which is associated with the down-regulation of OseIF3f. Further cytological observations of pollen development revealed that the OseIF3f-RNAi lines showed no obvious abnormalities at the male meiotic stage and the unicellular microspore stage. However, compared to the wild-type, OseIF3f-RNAi lines contained a higher percentage of arrested unicellular pollen at the bicellular stage and a higher percentage of arrested unicellular and bicellular pollen, and aborted pollen at the tricellular stage. These results indicate that OseiF3f plays a role in microgametogenesis.
关键词	pollen development microgametogenesis translation initiation eIF3f rice
学科领域	Plant Sciences
DOI	10.3389/fpls.2016.00532
收录类别	SCI
语种	英语
WOS关键词	POLLEN DEVELOPMENT ; GENE-EXPRESSION ; PROTEIN ; MEIOSIS ; ENCODES ; EIF3 ; ORTHOLOGUE ; REGULATOR ; REVEALS ; COMPLEX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000374731900001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2011CB944602, 2012CB910504]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25355
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Qi,Deng, Zhuyun,Gong, Chunyan,et al. The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Li, Qi,Deng, Zhuyun,Gong, Chunyan,&Wang, Tai.(2016).The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis.FRONTIERS IN PLANT SCIENCE,7.
MLA	Li, Qi,et al."The Rice Eukaryotic Translation Initiation Factor 3 Subunit f (OseIF3f) Is Involved in Microgametogenesis".FRONTIERS IN PLANT SCIENCE 7(2016).

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