NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development

doi:10.1371/journal.pgen.1005923

	NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development
	Cui, Xiaona 1; Guo, Zhiai ; Song, Lizhen ; Wang, Yanli 1; Cheng, Youfa
	2016
发表期刊	PLOS GENETICS
ISSN	1553-7404
卷号	12 期号:3
摘要	MOB1 protein is a core component of the Hippo signaling pathway in animals where it is involved in controlling tissue growth and tumor suppression. Plant MOB1 proteins display high sequence homology to animal MOB1 proteins, but little is known regarding their role in plant growth and development. Herein we report the critical roles of Arabidopsis MOB1 (AtMOB1A) in auxin-mediated development in Arabidopsis. We found that loss-of-function mutations in AtMOB1A completely eliminated the formation of cotyledons when combined with mutations in PINOID (PID), which encodes a Ser/Thr protein kinase that participates in auxin signaling and transport. We showed that atmob1a was fully rescued by its Drosophila counterpart, suggesting functional conservation. The atmob1a pid double mutants phenocopied several well-characterized mutant combinations that are defective in auxin biosynthesis or transport. Moreover, we demonstrated that atmob1a greatly enhanced several other known auxin mutants, suggesting that AtMOB1A plays a key role in auxin-mediated plant development. The atmob1a single mutant displayed defects in early embryogenesis and had shorter root and smaller flowers than wild type plants. AtMOB1A is uniformly expressed in embryos and suspensor cells during embryogenesis, consistent with its role in embryo development. AtMOB1A protein is localized to nucleus, cytoplasm, and associated to plasma membrane, suggesting that it plays roles in these subcellular localizations. Furthermore, we showed that disruption of AtMOB1A led to a reduced sensitivity to exogenous auxin. Our results demonstrated that AtMOB1A plays an important role in Arabidopsis development by promoting auxin signaling.
学科领域	Genetics & Heredity
DOI	10.1371/journal.pgen.1005923
收录类别	SCI
语种	英语
WOS关键词	HIPPO SIGNALING PATHWAY ; YUCCA FLAVIN MONOOXYGENASES ; D6 PROTEIN-KINASE ; CELL-PROLIFERATION ; TUMOR-SUPPRESSOR ; REGULATED PROTEIN ; ORGAN SIZE ; GROWTH ; GENE ; PHOSPHORYLATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373268900032
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; National Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [31171389, 91217310, 91017008, 31270330] ; One-hundred Talent Project of the Chinese Academy of SciencesChinese Academy of Sciences ; K.C. Wong Education Foundation, Hong Kong
作者邮箱	yfcheng@ibcas.ac.cn
作品OA属性	Green Published, gold, Green Submitted
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25360
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Xiaona,Guo, Zhiai,Song, Lizhen,et al. NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development[J]. PLOS GENETICS,2016,12(3).
APA	Cui, Xiaona,Guo, Zhiai,Song, Lizhen,Wang, Yanli,&Cheng, Youfa.(2016).NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development.PLOS GENETICS,12(3).
MLA	Cui, Xiaona,et al."NCP1/AtMOB1A Plays Key Roles in Auxin-Mediated Arabidopsis Development".PLOS GENETICS 12.3(2016).

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