Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii

doi:10.1111/tpj.13133

	Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii
	Yang, Hao 1; Yang, Ning ; Wang, Tai
	2016
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	85 期号:5 页码:660-674
摘要	In flowering plants, male germline fate is determined after asymmetric division of the haploid microspore. Daughter cells have distinct fates: the generative cell (GC) undergoes further mitosis to generate sperm cells (SCs), and the vegetative cell (VC) terminally differentiates. However, our understanding of the mechanisms underlying germline development remains limited. Histone variants and modifications define chromatin states, and contribute to establishing and maintaining cell identities by affecting gene expression. Here, we constructed a lily protein database, then extracted and detailed histone entries into a comprehensive lily histone database. We isolated large amounts of nuclei from VCs, GCs and SCs from lily, and profiled histone variants of all five histone families in all three cell types using proteomics approaches. We revealed 92 identities representing 32 histone variants: six for H1, 11 for H2A, eight for H2B, five for H3 and two for H4. Nine variants, including five H1, two H2B, one H3 and one H4 variant, specifically accumulated in GCs and SCs. We also detected H3 modification patterns in the three cell types. GCs and SCs had almost identical histone profiles and similar H3 modification patterns, which were significantly different from those of VCs. Our study also revealed the presence of multiple isoforms, and differential expression patterns between isoforms of a variant. The results suggest that differential histone programs between the germline and companion VCs may be established following the asymmetric division, and are important for identity establishment and differentiation of the male germline as well as the VC. Significance Statement Histone variants and modifications define chromatin states and contribute to establishing and maintaining cell identities by affecting gene expression. Using proteomics of lily pollen, we show that there are distinct histone profiles in the vegetative cell and in the generative cell and its progeny sperm cells. We propose that differential histone programmes are important for identity establishment and differentiation of the male germline and the vegetative cell.
关键词	histone program histone variant male germline sperm cells generative cells vegetative cells Lilium davidii
学科领域	Plant Sciences
DOI	10.1111/tpj.13133
收录类别	SCI
语种	英语
WOS关键词	MALE GAMETOPHYTE DEVELOPMENT ; MALE GAMETIC CELLS ; MASS-SPECTROMETRY ; SPERM CELLS ; GENERATIVE CELLS ; POSTTRANSLATIONAL MODIFICATIONS ; CONSTITUTIVE HETEROCHROMATIN ; QUANTITATIVE PROTEOMICS ; CHROMATIN MODIFICATIONS ; ARABIDOPSIS-THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000372274500007
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2012CB910504, 2013CB945101]
作者邮箱	twang@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25361
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Hao,Yang, Ning,Wang, Tai. Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii[J]. PLANT JOURNAL,2016,85(5):660-674.
APA	Yang, Hao,Yang, Ning,&Wang, Tai.(2016).Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii.PLANT JOURNAL,85(5),660-674.
MLA	Yang, Hao,et al."Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii".PLANT JOURNAL 85.5(2016):660-674.

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