Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

doi:10.1038/srep32717

	Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses
	Zhao, Qi 1; Suo, Jinwei 1; Chen, Sixue 2; Jin, Yudan 1; Ma, Xiaolin 1; Yin, Zepeng ; Zhang, Yuhong ; Wang, Tai3 ; Luo, Ji 4; Jin, Wenhai 4; Zhang, Xia 3; Zhou, Zhiqiang ; Dai, Shaojun 1
	2016
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	6
摘要	Soil alkalization severely affects crop growth and agricultural productivity. Alkali salts impose ionic, osmotic, and high pH stresses on plants. The alkali tolerance molecular mechanism in roots from halophyte Puccinellia tenuiflora is still unclear. Here, the changes associated with Na2CO3 tolerance in P. tenuiflora roots were assessed using physiological and iTRAQ-based quantitative proteomic analyses. We set up the first protein dataset in P. tenuiflora roots containing 2,671 non-redundant proteins. Our results showed that Na2CO3 slightly inhibited root growth, caused ROS accumulation, cell membrane damage, and ion imbalance, as well as reduction of transport and protein synthesis/turnover. The Na2CO3-responsive patterns of 72 proteins highlighted specific signaling and metabolic pathways in roots. Ca2+ signaling was activated to transmit alkali stress signals as inferred by the accumulation of calcium-binding proteins. Additionally, the activities of peroxidase and glutathione peroxidase, and the peroxiredoxin abundance were increased for ROS scavenging. Furthermore, ion toxicity was relieved through Na+ influx restriction and compartmentalization, and osmotic homeostasis reestablishment due to glycine betaine accumulation. Importantly, two transcription factors were increased for regulating specific alkali-responsive gene expression. Carbohydrate metabolism-related enzymes were increased for providing energy and carbon skeletons for cellular metabolism. All these provide new insights into alkali-tolerant mechanisms in roots.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep32717
收录类别	SCI
语种	英语
WOS关键词	STRESS-RESPONSIVE PROTEINS ; EXPRESSED SEQUENCE TAGS ; SALT-STRESS ; GENE-EXPRESSION ; ENVIRONMENTAL-STRESSES ; OVER-EXPRESSION ; SEEDLING ROOTS ; SALINE-ALKALI ; CONFERS SALT ; TOLERANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000382467100001
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270310] ; Fundamental Research Funds for the Central Universities, ChinaFundamental Research Funds for the Central Universities [2572014EA04] ; Special Fund for Forestry-Scientific Research in the Public Interest [201104066]
作者邮箱	zhiqiangzhou@hotmail.com ; daishaojun@hotmail.com
作品OA属性	gold, Green Published
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25367
专题	中科院植物分子生理学重点实验室
作者单位	1.Northeast Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Salinealkali Vegetat Ecol Restorat Oil Fi, Minist Educ, Harbin 150040, Peoples R China 3.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Genet Inst, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL 32610 USA 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.AB Sciex Asia Pacific Applicat Support Ctr, Shanghai 200233, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Qi,Suo, Jinwei,Chen, Sixue,et al. Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses[J]. SCIENTIFIC REPORTS,2016,6.
APA	Zhao, Qi.,Suo, Jinwei.,Chen, Sixue.,Jin, Yudan.,Ma, Xiaolin.,...&Dai, Shaojun.(2016).Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses.SCIENTIFIC REPORTS,6.
MLA	Zhao, Qi,et al."Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses".SCIENTIFIC REPORTS 6(2016).

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