Knowledge Management System Of Institute Of Botany,CAS
| RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway | |
Chan, Zhulong; Wang, Yanping; Cao, Minjie; Gong, Yuehua4; Mu, Zixin5; Wang, Haiqing6; Hu, Yuanlei7; Deng, Xin8 ; He, Xin-Jian; Zhu, Jian-Kang
| |
| 2016 | |
| Source Publication | NEW PHYTOLOGIST
 |
| ISSN | 0028-646X |
| Volume | 209Issue:4Pages:1527-1539 |
| Abstract | The C-REPEAT-BINDING FACTOR (CBF) pathway has important roles in plant responses to cold stress. How the CBF genes themselves are activated after cold acclimation remains poorly understood. In this study, we characterized cold tolerance of null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4), which encodes a protein that associates with RNA polymerases Pol V and Pol II, and is required for RNA-directed DNA methylation (RdDM) in Arabidopsis. The results showed that dysfunction of RDM4 reduced cold tolerance, as evidenced by decreased survival and increased electrolyte leakage. Mutation of RDM4 resulted in extensive transcriptomic reprogramming. CBFs and CBF regulon genes were down-regulated in rdm4 but not nrpe1 (the largest subunit of PolV) mutants, suggesting that the role of RDM4 in cold stress responses is independent of the RdDM pathway. Overexpression of RDM4 constitutively increased the expression of CBFs and regulon genes and decreased cold-induced membrane injury. A great proportion of genes affected by rdm4 overlapped with those affected by CBFs. Chromatin immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3. We present evidence of a considerable role for RDM4 in regulating gene expression at low temperature, including the CBF pathway in Arabidopsis. |
| Keyword | C-REPEAT-BINDING FACTOR (CBF) cold Pol II RNA-directed DNA methylation RNA-DIRECTED DNA METHYLATION 4 (RDM4) transcriptome |
| Subject Area | Plant Sciences |
| DOI | 10.1111/nph.13727 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | RESPONSIVE GENE-EXPRESSION ; LOW-TEMPERATURE ; TRANSCRIPTION FACTORS ; FREEZING TOLERANCE ; OXIDATIVE STRESS ; CIRCADIAN CLOCK ; ACCLIMATION ; THALIANA ; BINDING ; ROLES |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000373379800022 |
| Publisher | WILEY |
| Subtype | Article |
| Publication Place | HOBOKEN |
| EISSN | 1469-8137 |
| Funding Organization | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370302] ; Knowledge Innovative Key Program of the Chinese Academy of Sciences [54Y154761O01076, Y329631O0263] ; Chinese Academy of SciencesChinese Academy of Sciences ; US National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM059138] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM059138, R01GM070795] Funding Source: NIH RePORTER |
| Corresponding Author Email | zhulongch@wbgcas.cn ; jkzhu@purdue.edu |
| OA | Green Accepted, Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25381 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei Province, Peoples R China 2.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 4.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA 5.Yibin Univ, Coll Life Sci & Food Engn, Yibin 644000, Sichuan, Peoples R China 6.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 7.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 8.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 9.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 10.Natl Inst Biol Sci, Beijing 102206, Peoples R China |
| Recommended Citation GB/T 7714 | Chan, Zhulong,Wang, Yanping,Cao, Minjie,et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway[J]. NEW PHYTOLOGIST,2016,209(4):1527-1539. |
| APA | Chan, Zhulong.,Wang, Yanping.,Cao, Minjie.,Gong, Yuehua.,Mu, Zixin.,...&Zhu, Jian-Kang.(2016).RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway.NEW PHYTOLOGIST,209(4),1527-1539. |
| MLA | Chan, Zhulong,et al."RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway".NEW PHYTOLOGIST 209.4(2016):1527-1539. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Chan-2016-RDM4 modul（1587KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment