RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway

doi:10.1111/nph.13727

	RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway
	Chan, Zhulong ; Wang, Yanping ; Cao, Minjie ; Gong, Yuehua 4; Mu, Zixin 5; Wang, Haiqing 6; Hu, Yuanlei 7; Deng, Xin8 ; He, Xin-Jian ; Zhu, Jian-Kang
	2016
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	209 期号:4 页码:1527-1539
摘要	The C-REPEAT-BINDING FACTOR (CBF) pathway has important roles in plant responses to cold stress. How the CBF genes themselves are activated after cold acclimation remains poorly understood. In this study, we characterized cold tolerance of null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4), which encodes a protein that associates with RNA polymerases Pol V and Pol II, and is required for RNA-directed DNA methylation (RdDM) in Arabidopsis. The results showed that dysfunction of RDM4 reduced cold tolerance, as evidenced by decreased survival and increased electrolyte leakage. Mutation of RDM4 resulted in extensive transcriptomic reprogramming. CBFs and CBF regulon genes were down-regulated in rdm4 but not nrpe1 (the largest subunit of PolV) mutants, suggesting that the role of RDM4 in cold stress responses is independent of the RdDM pathway. Overexpression of RDM4 constitutively increased the expression of CBFs and regulon genes and decreased cold-induced membrane injury. A great proportion of genes affected by rdm4 overlapped with those affected by CBFs. Chromatin immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3. We present evidence of a considerable role for RDM4 in regulating gene expression at low temperature, including the CBF pathway in Arabidopsis.
关键词	C-REPEAT-BINDING FACTOR (CBF) cold Pol II RNA-directed DNA methylation RNA-DIRECTED DNA METHYLATION 4 (RDM4) transcriptome
学科领域	Plant Sciences
DOI	10.1111/nph.13727
收录类别	SCI
语种	英语
WOS关键词	RESPONSIVE GENE-EXPRESSION ; LOW-TEMPERATURE ; TRANSCRIPTION FACTORS ; FREEZING TOLERANCE ; OXIDATIVE STRESS ; CIRCADIAN CLOCK ; ACCLIMATION ; THALIANA ; BINDING ; ROLES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000373379800022
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1469-8137
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370302] ; Knowledge Innovative Key Program of the Chinese Academy of Sciences [54Y154761O01076, Y329631O0263] ; Chinese Academy of SciencesChinese Academy of Sciences ; US National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM059138] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM059138, R01GM070795] Funding Source: NIH RePORTER
作者邮箱	zhulongch@wbgcas.cn ; jkzhu@purdue.edu
作品OA属性	Green Accepted, Bronze
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25381
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei Province, Peoples R China 2.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 4.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA 5.Yibin Univ, Coll Life Sci & Food Engn, Yibin 644000, Sichuan, Peoples R China 6.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 7.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 8.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 9.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 10.Natl Inst Biol Sci, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Chan, Zhulong,Wang, Yanping,Cao, Minjie,et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway[J]. NEW PHYTOLOGIST,2016,209(4):1527-1539.
APA	Chan, Zhulong.,Wang, Yanping.,Cao, Minjie.,Gong, Yuehua.,Mu, Zixin.,...&Zhu, Jian-Kang.(2016).RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway.NEW PHYTOLOGIST,209(4),1527-1539.
MLA	Chan, Zhulong,et al."RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway".NEW PHYTOLOGIST 209.4(2016):1527-1539.

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