Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum

doi:10.1016/j.ijfoodmicro.2015.05.007

	Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum
	Zong, Yuanyuan 1; Li, Boqiang ; Tian, Shiping
	2015
发表期刊	INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN	0168-1605
卷号	206 页码:102-108
摘要	Patulin, a potent mycotoxin which can cause serious health concerns, is mainly produced in foods by Penicillium expansum. Environmental factors play important roles in regulating biosynthesis of mycotoxins; however, information about the effects of environmental factors on patulin production and the involved mechanisms in P. expaasum is limited. Here, we investigated the effects of different carbon (C) and nitrogen (N) sources, and ambient pH on patulin production in three P. expansum strains T01, M1 and Pe21, and the expression profile of 15 genes involved in patulin biosynthetic pathway. It was found that C and N sources and pH had great influence on patulin production in P. expansum. In general, patulin production of all three P. expansum strains showed similar trends under different C and N sources and pH conditions, though there were some differences in the optimal conditions among these strains. Glucose-containing sugars, complex N sources, and acidic conditions were favorable conditions for patulin production. The results of RT-qPCR showed that the relative expressions of most of the patulin genes were up-regulated under patulin-permissive conditions, indicating that patulin biosynthesis was mainly regulated at transcriptional level by these environmental factors. These findings will provide useful information to better understand the regulation mechanisms of patulin biosynthesis, and be helpful in developing effective means for controlling patulin contamination. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Penicillium expansum Patulin biosynthesis Carbon source Nitrogen source Ambient pH
学科领域	Food Science & Technology ; Microbiology
DOI	10.1016/j.ijfoodmicro.2015.05.007
收录类别	SCI
语种	英语
WOS关键词	SECONDARY METABOLISM ; ASPERGILLUS-PARASITICUS ; BIOSYNTHESIS ; AFLATOXIN ; STERIGMATOCYSTIN ; MECHANISMS ; REPRESSION ; GROWTH ; APPLE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000358100500015
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-3460
资助机构	National High Technology Research (863) Program of China [2012AA101607] ; National Natural Science Foundation of China [31171765, 31030051]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：77[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25573
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zong, Yuanyuan,Li, Boqiang,Tian, Shiping. Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2015,206:102-108.
APA	Zong, Yuanyuan,Li, Boqiang,&Tian, Shiping.(2015).Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,206,102-108.
MLA	Zong, Yuanyuan,et al."Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 206(2015):102-108.

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