Integrated and comparative proteomics of high-oil and high-protein soybean seeds

doi:10.1016/j.foodchem.2014.09.035

	Integrated and comparative proteomics of high-oil and high-protein soybean seeds
	Xu, Xiu Ping 1; Liu, Hui 2; Tian, Lihong ; Dong, Xiang Bai ; Shen, Shi Hua 2; Qu, Le Qing
	2015
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	172 页码:105-116
摘要	We analysed the global protein expression in seeds of a high-oil soybean cultivar (Jiyu 73, JY73) by proteomics. More than 700 protein spots were detected and 363 protein spots were successfully identified. Comparison of the protein profile of JY73 with that of a high-protein cultivar (Zhonghuang 13, ZH13) revealed 40 differentially expressed proteins, including oil synthesis, redox/stress, hydrolysis and storage-related proteins. All redox/stress proteins were less or not expressed in JY73, whereas the expression of the major storage proteins, nitrogen and carbon metabolism-related proteins was higher in ZH13. Biochemical analysis of JY73 revealed that it was in a low oxidation state, with a high content of polyunsaturated fatty acids and vitamin E. Vitamin E was more active than antioxidant enzymes and protected the soybean seed in a lower oxidation state. The characteristics of high oil and high protein in soybean, we revealed, might provide a reference for soybean nutrition and soybean breeding. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Antioxidant Comparative analysis Global protein expression Proteomics Soybean
学科领域	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
DOI	10.1016/j.foodchem.2014.09.035
收录类别	SCI
语种	英语
WOS关键词	CYTOSOLIC GLUTAMINE-SYNTHETASE ; STORAGE PROTEINS ; LIPID-PEROXIDATION ; BETA-CONGLYCININ ; GLYCININ ; ACCUMULATION ; EXPRESSION ; SEQUENCE ; MOBILIZATION ; GERMINATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000345207200015
出版者	ELSEVIER SCI LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7072
资助机构	National Program of Transgenic Variety Development of China [2014ZX08012-002]
作者邮箱	lqqu@ibcas.ac.cn
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25575
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiu Ping,Liu, Hui,Tian, Lihong,et al. Integrated and comparative proteomics of high-oil and high-protein soybean seeds[J]. FOOD CHEMISTRY,2015,172:105-116.
APA	Xu, Xiu Ping,Liu, Hui,Tian, Lihong,Dong, Xiang Bai,Shen, Shi Hua,&Qu, Le Qing.(2015).Integrated and comparative proteomics of high-oil and high-protein soybean seeds.FOOD CHEMISTRY,172,105-116.
MLA	Xu, Xiu Ping,et al."Integrated and comparative proteomics of high-oil and high-protein soybean seeds".FOOD CHEMISTRY 172(2015):105-116.

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