Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma

doi:10.1515/biolog-2015-0122

	Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma
	Hao, He ; Pu, Gao Bin 1; Lei, Cai Yan 2; Ye, He Chun 3; Wang, Hong
	2015
发表期刊	BIOLOGIA
ISSN	0006-3088
卷号	70 期号:8 页码:1053-1062
摘要	Callus cultures of Arnebia euchroma (Royle) Johnst can produce considerable amounts of shikonin derivatives, which are shown to possess a wide spectrum of pharmaceutical properties, such as antimicrobial, anticancer, antioxidative, immune regulative and anti-inflammatory. To explore the possible regulation mechanism of shikonin derivatives biosynthesis, the effects of ammonium ion (NH4+) on cell growth and formation of shikonin derivatives in callus cultures of A. euchroma were investigated in this paper. The main results include: When A. euchroma callus were cultured in medium with different concentrations of NH4+ (0 mM, 7.02 mM, 14.04 mM), the fresh weight of the cell cultured in 7.02 mM NH4+ was higher than those in 0 mM and 14.04 mM NH4+, indicating that NH4+ is necessary for the cell growth of A. euchroma callus cultures, while excessive NH4+ concentration (14.04 mM) could rather inhibit the cell growth of A. euchroma. The quantitative analysis of the seven main naphthoquinone compounds showed that the contents of most of the shikonin derivatives (including the bioactive components acetylshikonin and beta,beta-dimethyl-acrylshikonin) of the Red Strain cultured in 0 mM NH4+ condition were higher than those in other NH4+ conditions (7.02 mM, 14.04 mM) at most of the sample points, suggesting that NH4+ essentially plays an unfavorable role in the biosynthesis of shikonin derivatives. The analysis of quantitative real-time RTPCR showed that a higher NH4+ concentration inhibited the expression of key enzyme genes (such as AePGT) of shikonin derivatives biosynthesis, while -NH4+ condition was conducive to the expression of such genes. In sum, our results show that NH4+ is necessary for the cell growth of A. euchroma, while unnecessary, or even adversary to the biosynthesis of shikonin derivatives.
关键词	Arnebia euchroma callus shikonin derivatives ammonium ion gene expression
学科领域	Biology
DOI	10.1515/biolog-2015-0122
收录类别	SCI
语种	英语
WOS关键词	LITHOSPERMUM-ERYTHRORHIZON ; GENE-EXPRESSION ; CDNA CLONING ; CULTURES ; PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000361772300008
出版者	WALTER DE GRUYTER GMBH
文献子类	Article
出版地	BERLIN
EISSN	1336-9563
资助机构	National Natural Science Foundation of China [61173098, 61379081]
作者邮箱	hwang@ucas.ac.cn
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25576
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Shandong Univ Tradit Chinese Med, Jinan 250022, Peoples R China 3.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Hao, He,Pu, Gao Bin,Lei, Cai Yan,et al. Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma[J]. BIOLOGIA,2015,70(8):1053-1062.
APA	Hao, He,Pu, Gao Bin,Lei, Cai Yan,Ye, He Chun,&Wang, Hong.(2015).Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma.BIOLOGIA,70(8),1053-1062.
MLA	Hao, He,et al."Effects of ammonium ion on cell growth and biosynthesis of shikonin derivatives in callus tissues of Arnebia euchroma".BIOLOGIA 70.8(2015):1053-1062.

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