Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth

doi:10.1105/tpc.114.135400

	Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth
	Zhou, Zhenzhen ; Shi, Haifan ; Chen, Binqing ; Zhang, Ruihui 1; Huang, Shanjin 1; Fu, Ying
	2015
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	27 期号:4 页码:1140-1161
摘要	Pollen tubes deliver sperms to the ovule for fertilization via tip growth. The rapid turnover of F-actin in pollen tube tips plays an important role in this process. In this study, we demonstrate that Arabidopsis thaliana RIC1, a member of the ROP-interactive CRIB motif-containing protein family, regulates pollen tube growth via its F-actin severing activity. Knockout of RIC1 enhanced pollen tube elongation, while overexpression of RIC1 dramatically reduced tube growth. Pharmacological analysis indicated that RIC1 affected F-actin dynamics in pollen tubes. In vitro biochemical assays revealed that RIC1 directly bound and severed F-actin in the presence of Ca2+ in addition to interfering with F-actin turnover by capping F-actin at the barbed ends. In vivo, RIC1 localized primarily to the apical plasma membrane (PM) of pollen tubes. The level of RIC1 at the apical PM oscillated during pollen tube growth. The frequency of F-actin severing at the apex was notably decreased in ric1-1 pollen tubes but was increased in pollen tubes overexpressing RIC1. We propose that RIC1 regulates F-actin dynamics at the apical PM as well as the cytosol by severing F-actin and capping the barbed ends in the cytoplasm, establishing a novel mechanism that underlies the regulation of pollen tube growth.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1105/tpc.114.135400
收录类别	SCI
语种	英语
WOS关键词	CELL-GROWTH ; RHO-GTPASE ; TIP GROWTH ; BINDING PROTEINS ; SPATIAL CONTROL ; DYNAMICS ; CYTOSKELETON ; GERMINATION ; POLARITY ; PATHWAYS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000354822800017
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-298X
资助机构	National Basic Research Program of China [2012CB114200] ; Natural Science Foundation of China [31361140354]
作者邮箱	yingfu@cau.edu.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：54[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25589
专题	中科院植物分子生理学重点实验室
作者单位	1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Zhenzhen,Shi, Haifan,Chen, Binqing,et al. Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth[J]. PLANT CELL,2015,27(4):1140-1161.
APA	Zhou, Zhenzhen,Shi, Haifan,Chen, Binqing,Zhang, Ruihui,Huang, Shanjin,&Fu, Ying.(2015).Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth.PLANT CELL,27(4),1140-1161.
MLA	Zhou, Zhenzhen,et al."Arabidopsis RIC1 Severs Actin Filaments at the Apex to Regulate Pollen Tube Growth".PLANT CELL 27.4(2015):1140-1161.

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