Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells

doi:10.1016/j.molp.2015.04.005

	Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells
	Wang, Li 1,7; Li, Hong 1,7; Lv, Xueqin 2; Chen, Tong ; Li, Ruili 2; Xue, Yiqun ; Jiang, Jianjun 3; Jin, Biao 1; Baluska, Frantisek 4; Samaj, Jozef 5; Wang, Xuelu 3,6; Lin, Jinxing 2
	2015
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	8 期号:9 页码:1334-1349
摘要	The major brassinosteroid (BR) receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1) plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRI1. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFlot1-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFlot1 amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bri1-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.
关键词	BRI1 BR signaling endocytosis membrane microdomains spatiotemporal dynamics
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2015.04.005
收录类别	SCI
语种	英语
WOS关键词	CLATHRIN-INDEPENDENT ENDOCYTOSIS ; CROSS-CORRELATION SPECTROSCOPY ; DETERGENT-RESISTANT MEMBRANES ; SINGLE-MOLECULE ; PLASMA-MEMBRANE ; EGF RECEPTOR ; PLANT-CELLS ; BRASSINOSTEROID RECEPTORS ; DEPENDENT ENDOCYTOSIS ; FLUORESCENT MARKERS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000360980300005
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	111 project [B13007] ; Major Science Foundation of Ministry of Education of China [313008] ; National Natural Science Foundation of China [91317302, 31271300, 31401149] ; State Key Laboratory of Genetic Engineering, Fudan University ; [ED0007/01/01]
作者邮箱	linjinxing@bjfu.edu.cn
作品OA属性	Bronze
引用统计	被引频次：83[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25610
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Peoples R China 3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 4.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China 5.Fudan Univ, Sch Life Sci, Inst Plant Biol, Shanghai 200433, Peoples R China 6.Univ Bonn, Inst Cellular & Mol Bot, D-53115 Bonn, Germany 7.Palacky Univ, Dept Cell Biol, Olomouc 78371, Czech Republic 8.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Li, Hong,Lv, Xueqin,et al. Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells[J]. MOLECULAR PLANT,2015,8(9):1334-1349.
APA	Wang, Li.,Li, Hong.,Lv, Xueqin.,Chen, Tong.,Li, Ruili.,...&Lin, Jinxing.(2015).Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells.MOLECULAR PLANT,8(9),1334-1349.
MLA	Wang, Li,et al."Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells".MOLECULAR PLANT 8.9(2015):1334-1349.

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