TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance

doi:10.1007/s11105-014-0737-x

	TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance
	Ma, Qing-Hu ; Liu, Yun-Chao
	2015
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	33 期号:1 页码:143-152
摘要	Dirigent is proposed to be involved in either lignan or lignin biosynthesis. In the present study, sequences for dirigent and dirigent-like proteins from the wheat genome were analyzed. We obtained wheat dirigent (TaDIR13), and dirigent-like (TaDIR4 and TaJA1) recombinant proteins used for biochemical assays. It was shown that TaDIR13 could effectively direct coniferyl alcohol coupling into (+)-pinoresinol. In contrast, TaDIR4 and TaJA1 did not exhibit this activity. In accordance with this role of TaDIR13, overexpression of TaDIR13 in transgenic tobacco increased total lignan accumulation. Lignan extracts from TaDIR13 transgenic plants had enhanced antibacterial effects to Pseudomonas syringae. Furthermore, these plants did show strong resistance to P. syringae and Phytophthora parasitica. On the contrary, TaDIR4 transgenics did not show noticeable changes in either lignan accumulation or pathogen resistance. Overexpression of TaJA1 did not increase lignan accumulation either. Unlike TaDIR4 plants though, TaJA1 plants exhibited high pathogen resistance; yet, this was achieved through its jacalin-related lectins domain and not its dirigent domain. Lignin content and gene expression were not affected in all transgenic plants. Interestingly, expression of the pinoresinol-lariciresinol reductase gene (NtPLR) was increased in TaDIR13 transgenic plant; this is apparently owing to a favored metabolic flux from coniferyl alcohol into the lignan pathway. These data collectively suggest that TaDIR13 is mainly involved in regulating lignan biosynthesis which, in turn, responsible for the observed roles in pathogen resistance. TaDIR4 and TaJA1, both being dirigent-like proteins, have little functional overlap with dirigent proteins.
关键词	Dirigent Lignan Lignin Metabolic flux Pathogen resistance Triticum aestivum
学科领域	Biochemical Research Methods ; Plant Sciences
DOI	10.1007/s11105-014-0737-x
收录类别	SCI
语种	英语
WOS关键词	PINORESINOL-LARICIRESINOL REDUCTASE ; CINNAMOYL-COA REDUCTASE ; SPRUCE PICEA SPP. ; GENE-EXPRESSION ; CONIFER DEFENSE ; OVEREXPRESSION ; TRANSCRIPTS ; PHYLOGENY ; DISCOVERY ; DISEASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000347953500011
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1572-9818
资助机构	National Natural Science Foundation of China [31170277, 31370336, 31070235] ; Natural Science Foundation of Beijing [5122024] ; Chinese Academy of Sciences
作者邮箱	mqh@ibcas.ac.cn
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25621
专题	中科院北方资源植物重点实验室
作者单位	1.[Ma, Qing-Hu 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ma, Qing-Hu,Liu, Yun-Chao. TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(1):143-152.
APA	Ma, Qing-Hu,&Liu, Yun-Chao.(2015).TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance.PLANT MOLECULAR BIOLOGY REPORTER,33(1),143-152.
MLA	Ma, Qing-Hu,et al."TaDIR13, a Dirigent Protein from Wheat, Promotes Lignan Biosynthesis and Enhances Pathogen Resistance".PLANT MOLECULAR BIOLOGY REPORTER 33.1(2015):143-152.

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