Abundance and Diversity of RuBisCO Genes Responsible for CO2 Fixation in Arid Soils of Northwest China

doi:10.1016/S1002-0160(14)60085-0

	Abundance and Diversity of RuBisCO Genes Responsible for CO2 Fixation in Arid Soils of Northwest China
	Tang Zhi-Xi ; Fan Fen-Liang ; Wan Yun-Fan ; Wei Wei ; Lai Li-Ming
	2015
发表期刊	PEDOSPHERE
ISSN	1002-0160
卷号	25 期号:1 页码:150-159
摘要	Arid soils where water and nutrients are scarce occupy over 30% of the Earth's total surface. However, the microbial autotrophy in the harsh environments remains largely unexplored. In this study, the abundance and diversity of autotrophic bacteria were investigated, by quantifying and profiling the large subunit genes of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) form I (cbbL) responsible for CO2 fixation, in the arid soils under three typical plant types (Haloxylon ammodendron, Cleistogenes chinensis, and Reaumuria soongorica) in Northwest China. The bacterial communities in the soils were also characterized using the 16S rRNA gene. Abundance of red-like autotrophic bacteria ranged from 3.94 x 10(5) to 1.51 x 10(6) copies g(-1) dry soil and those of green-like autotrophic bacteria ranged from 1.15 x 10(6) to 2.08 x 10(6) copies g(-1) dry soil. Abundance of both red- and green-like autotrophic bacteria did not significantly differ among the soils under different plant. types. The autotrophic bacteria identified with the cbbL gene primer were mainly affiliated with Alphaproteobacteria, Betaproteobacteria and an uncultured bacterial group, which were not detected in the 16S rRNA library. In addition, 25.9% and 8.1% of the 16S rRNA genes were affiliated with Cyanobacteria in the soils under H. ammodendron and R. soongorica, respectively. However, no Cyanobacteria-affiliated cbbL genes were detected in the same soils. The results suggested that microbial autotrophic CO2 fixation might be significant in the carbon cycling of arid soils, which warrants further exploration.
关键词	autotrophic bacteria carbon cycling cbbL harsh environments real-time polymerase chain reaction
学科领域	Soil Science
DOI	10.1016/S1002-0160(14)60085-0
收录类别	SCI
语种	英语
WOS关键词	LARGE-SUBUNIT GENES ; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ; COMMUNITY STRUCTURE ; CARBON ; SEQUESTRATION ; IMPACTS ; CBBL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000350011700015
出版者	SCIENCE PRESS
文献子类	Article
出版地	BEIJING
EISSN	2210-5107
资助机构	National Basic Research Program (973 Program) of China [2009-CB825103] ; National Natural Science Foundation of China [40901119]
作者邮箱	flfan@caas.ac.cn
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25626
专题	植被与环境变化国家重点实验室
作者单位	1.[Tang Zhi-Xi 2.Wei Wei 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 4.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Crop Nutr & Fertilizat, Beijing 100081, Peoples R China 5.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Minist Agr, Key Lab Agroenvironm & Climate Change, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Tang Zhi-Xi,Fan Fen-Liang,Wan Yun-Fan,et al. Abundance and Diversity of RuBisCO Genes Responsible for CO2 Fixation in Arid Soils of Northwest China[J]. PEDOSPHERE,2015,25(1):150-159.
APA	Tang Zhi-Xi,Fan Fen-Liang,Wan Yun-Fan,Wei Wei,&Lai Li-Ming.(2015).Abundance and Diversity of RuBisCO Genes Responsible for CO2 Fixation in Arid Soils of Northwest China.PEDOSPHERE,25(1),150-159.
MLA	Tang Zhi-Xi,et al."Abundance and Diversity of RuBisCO Genes Responsible for CO2 Fixation in Arid Soils of Northwest China".PEDOSPHERE 25.1(2015):150-159.

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