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A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis | |
Fang, Zhengwu1; Zhang, Xiaohong; Gao, Jinfeng; Wang, Pengke; Xu, Xiaoyu; Liu, Zhixiong1; Shen, Shihua2; Feng, Baili | |
2015 | |
发表期刊 | PLANT MOLECULAR BIOLOGY REPORTER |
ISSN | 0735-9640 |
卷号 | 33期号:5页码:1510-1525 |
摘要 | CBF/DREB transcription factors play essential roles in plant stress signaling transduction pathway. We isolated and identified a CBF/DREB homologous gene, FeDREB1, from Fagopyrum esculentum. Protein sequence alignment and phylogenetic analyses revealed that the FeDREB1 was grouped into the DREB (A-1) lineage. Moreover, subcellular localization observations suggested that FeDREB1 localizes in the nucleus. Yeast one-hybrid assays showed that FeDREB1 protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast. These results further suggested that the FeRDEB1 protein was a CBF/DREB transcription factor. Expression analysis revealed that the transcript levels of the FeRDEB1 gene increased rapidly following low-/high-temperature treatment, drought stress, and exogenous ABA treatment. Over-expression of the FeDREB1 gene significantly enhanced the drought and freezing tolerance of transgenic Arabidopsis but resulted in its growth retardation. Moreover, the transgenic Arabidopsis lines were also highly sensitive to ABA application. Digital gene expression profiling (DGE) analysis indicated that increased transcript levels of many ABA-independent/-dependent stress-responsive genes in 35S::FeRDEB1 transgenic Arabidopsis, revealing that the FeDREB1 may participate in an ABA-dependent/-independent pathways. |
关键词 | Buckwheat CBF/DREB Abscisic acid (ABA) Freezing and drought tolerance |
学科领域 | Biochemical Research Methods ; Plant Sciences |
DOI | 10.1007/s11105-015-0851-4 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | COLD-RESPONSE PATHWAY ; LOW-TEMPERATURE ; FUNCTIONAL-ANALYSIS ; SALT TOLERANCE ; STRESS TOLERANCE ; ABSCISIC-ACID ; EXPRESSION ; DEHYDRATION ; RICE ; ELEMENT |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000360392800028 |
出版者 | SPRINGER |
文献子类 | Article |
出版地 | NEW YORK |
EISSN | 1572-9818 |
资助机构 | National Nature Science Foundation of China [31071472] ; Special Fund for Agro-scientific Research in the Public Interest of China [201303008] |
作者邮箱 | fengbaili@nwsuaf.edu.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25627 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shanxi, Peoples R China 2.Yangtze Univ, Inst Crop Genet & Breeding, Coll Agr, Jingzhou 434025, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100081, Peoples R China |
推荐引用方式 GB/T 7714 | Fang, Zhengwu,Zhang, Xiaohong,Gao, Jinfeng,et al. A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(5):1510-1525. |
APA | Fang, Zhengwu.,Zhang, Xiaohong.,Gao, Jinfeng.,Wang, Pengke.,Xu, Xiaoyu.,...&Feng, Baili.(2015).A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis.PLANT MOLECULAR BIOLOGY REPORTER,33(5),1510-1525. |
MLA | Fang, Zhengwu,et al."A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis".PLANT MOLECULAR BIOLOGY REPORTER 33.5(2015):1510-1525. |
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