Mechanism of H2O2-induced oxidative stress regulating viability and biocontrol ability of Rhodotorula glutinis

doi:10.1016/j.ijfoodmicro.2014.10.025

	Mechanism of H2O2-induced oxidative stress regulating viability and biocontrol ability of Rhodotorula glutinis
	Chen, Jian 1; Li, Boqiang ; Qin, Guozheng; Tian, Shiping1
	2015
发表期刊	INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
ISSN	0168-1605
卷号	193 页码:152-158
摘要	The use of antagonistic yeasts to control postharvest pathogens is a promising alternative to fungicides. The effectiveness of the antagonists against fungal pathogens is greatly dependent on their viability, which is usually mediated by reactive oxygen species (ROS). Here, we investigated the effects of H2O2-induced oxidative stress on the viability and biocontrol efficacy of Rhodotorula glutinis and, using flow cytometric analysis, observed the changes of ROS accumulation and apoptosis in the yeast cells with or without H2O2 treatment We found that the viability of R. glutinis decreased in a time-and dose-dependent manner under H2O2-induced oxidative stress. Compared to the control, yeast cells exposed to oxidative stress exhibited more accumulation of ROS and higher levels of protein oxidative damage, but showed lower efficacy for biocontrol of Penicillium expansum causing blue mold rot on peach fruit. The results indicate that apoptosis is a main cause of the cell viability loss in R. glutinis, which is attributed to ROS accumulation under oxidative stress. These findings offer a plausible explanation that oxidative stress affects biocontrol efficacy of R. glutinis via regulating its viability and cell apoptosis. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Rhodotorula glutinis Oxidative stress ROS Apoptosis Biocontrol efficacy
学科领域	Food Science & Technology ; Microbiology
DOI	10.1016/j.ijfoodmicro.2014.10.025
收录类别	SCI
语种	英语
WOS关键词	POSTHARVEST BIOLOGICAL-CONTROL ; ANTAGONISTIC YEASTS ; CRYPTOCOCCUS-LAURENTII ; PENICILLIUM-EXPANSUM ; INDUCED APOPTOSIS ; DISEASES ; FRUIT ; EFFICACY ; TOLERANCE ; TREHALOSE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000347510600020
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-3460
资助机构	National Natural Science Foundation of China [31172005, 31030051] ; National High Technology Research (863) Program of China [2012AA101607]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25631
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jian,Li, Boqiang,Qin, Guozheng,et al. Mechanism of H2O2-induced oxidative stress regulating viability and biocontrol ability of Rhodotorula glutinis[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2015,193:152-158.
APA	Chen, Jian,Li, Boqiang,Qin, Guozheng,&Tian, Shiping.(2015).Mechanism of H2O2-induced oxidative stress regulating viability and biocontrol ability of Rhodotorula glutinis.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,193,152-158.
MLA	Chen, Jian,et al."Mechanism of H2O2-induced oxidative stress regulating viability and biocontrol ability of Rhodotorula glutinis".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 193(2015):152-158.

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