The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis

doi:10.1111/tpj.12807

IB-CAS > 中科院光生物学重点实验室

	The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis
	Fan, Xiangyuan 1; Zhang, Jiao ; Li, Wenjing ; Peng, Lianwei
	2015
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	82 期号:2 页码:221-231
摘要	The chloroplast NADH dehydrogenase-like (NDH) complex is involved in cyclic electron transport around photosystemI (PSI) and chlororespiration. Although the NDH complex was discovered more than 20years ago, its low abundance and fragile nature render it recalcitrant to analysis, and it is thought that some of its subunits remain to be identified. Here, we identified the NDH subunit NdhV that readily disassociates from the NDH complex in the presence of detergent, salt and alkaline solutions. The Arabidopsis ndhv mutant is partially defective in the accumulation of NDH subcomplexA (SubA) and SubE, resulting in impaired NDH activity. NdhV was mainly detected in the wild-type thylakoid membrane, and its accumulation in thylakoids strictly depended on the presence of the NDH complex. Quantitative immunoblot analysis revealed that NdhV and NdhN occur at close to equimolar concentrations. Furthermore, several NDH subunits were co-immunopurified with NdhV using a combination of chemical crosslinking and an affinity chromatography assay. These data indicate that NdhV is an intrinsic subunit of NDH. We found that NdhV did not directly affect NDH activity, but that NDH SubA and SubE were more rapidly degraded in ndhv than in the wild type under high-light treatment. We propose that NdhV is an NDH subunit that stabilizes this complex, especially under high-light conditions. Significance Statement This work identifies the chloroplast NADH dehydrogenase-like (NDH) subunit NdhV, which is located on the most fragile part of the NDH complex. NdhV interacts with and stabilizes the NDH subcomplexes A and E, especially under high-light conditions.
关键词	NADH dehydrogenase-like complex cyclic electron transport Arabidopsis chloroplast stabilization
学科领域	Plant Sciences
DOI	10.1111/tpj.12807
收录类别	SCI
语种	英语
WOS关键词	CYCLIC ELECTRON FLOW ; DOMAIN-LIKE FOLD ; PHOTOSYSTEM-I ; NAD(P)H DEHYDROGENASE ; SUPERCOMPLEX FORMATION ; EFFICIENT OPERATION ; IDENTIFICATION ; OXIDOREDUCTASE ; PROTEINS ; PHOTOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000352581600004
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Foundation of China [31270289] ; Chinese Academy of Sciences
作者邮箱	penglianwei@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25703
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fan, Xiangyuan,Zhang, Jiao,Li, Wenjing,et al. The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis[J]. PLANT JOURNAL,2015,82(2):221-231.
APA	Fan, Xiangyuan,Zhang, Jiao,Li, Wenjing,&Peng, Lianwei.(2015).The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis.PLANT JOURNAL,82(2),221-231.
MLA	Fan, Xiangyuan,et al."The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis".PLANT JOURNAL 82.2(2015):221-231.

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