Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways

doi:10.1074/mcp.M114.047183

	Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways
	Choudhary, Mani Kant ; Nomura, Yuko 2; Wang, Lei3 ; Nakagami, Hirofumi 2; Somers, David E.
	2015
发表期刊	MOLECULAR & CELLULAR PROTEOMICS
ISSN	1535-9476
卷号	14 期号:8 页码:2243-2260
摘要	The circadian clock provides adaptive advantages to an organism, resulting in increased fitness and survival. The phosphorylation events that regulate circadian-dependent signaling and the processes which post-translationally respond to clock-gated signals are largely unknown. To better elucidate post-translational events tied to the circadian system we carried out a survey of circadian-regulated protein phosphorylation events in Arabidopsis seedlings. A large-scale mass spectrometry-based quantitative phosphoproteomics approach employing TiO2-based phosphopeptide enrichment techniques identified and quantified 1586 phosphopeptides on 1080 protein groups. A total of 102 phosphopeptides displayed significant changes in abundance, enabling the identification of specific patterns of response to circadian rhythms. Our approach was sensitive enough to quantitate oscillations in the phosphorylation of low abundance clock proteins (EARLY FLOWERING4; ELF4 and PSEUDORESPONSE REGULATOR3; PRR3) as well as other transcription factors and kinases. During constant light, extensive cyclic changes in phosphorylation status occurred in critical regulators, implicating direct or indirect regulation by the circadian system. These included proteins influencing transcriptional regulation, translation, metabolism, stress and phytohormones-mediated responses. We validated our analysis using the elf4-211 allele, in which an S45L transition removes the phosphorylation herein identified. We show that removal of this phosphorylatable site diminishes interaction with EARLY FLOWERING3 (ELF3), a key partner in a tripartite evening complex required for circadian cycling. elf4-211 lengthens period, which increases with increasing temperature, relative to the wild type, resulting in a more stable temperature compensation of circadian period over a wider temperature range.
学科领域	Biochemical Research Methods
DOI	10.1074/mcp.M114.047183
收录类别	SCI
语种	英语
WOS关键词	SUCROSE-PHOSPHATE SYNTHASE ; NITRATE REDUCTASE-ACTIVITY ; PROTEIN-KINASE CK2 ; POSTTRANSLATIONAL REGULATION ; PHOSPHORYLATION SITES ; TRANSDUCTION PATHWAY ; RESPONSIVE GENES ; EXPRESSION ; LIGHT ; RHYTHMS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000358837400015
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
文献子类	Article
出版地	ROCKVILLE
EISSN	1535-9484
资助机构	National Institutes of Health [R01GM093285] ; World Class University Program of South Korea [R31-2008-000-10105-0] ; NRF ; MEST ; JSPS KAKENHI [24688007, 26650106] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM093285] Funding Source: NIH RePORTER ; Grants-in-Aid for Scientific Research [24688007, 26650106] Funding Source: KAKEN
作者邮箱	somers.24@osu.edu
作品OA属性	Green Published, hybrid
引用统计	被引频次：76[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25705
专题	中科院植物分子生理学重点实验室
作者单位	1.[Choudhary, Mani Kant; Wang, Lei; Somers, David E.] POSTECH, Div Integrat Biosci & Biotechnol, Pohang 790784, Kyungbuk, South Korea 2.Wang, Lei; Somers, David E.] Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA 3.RIKEN Ctr Sustainable Resource Sci CSRS, Plant Prote Res Unit, Yokohama, Kanagawa 2300045, Japan 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Phys, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Choudhary, Mani Kant,Nomura, Yuko,Wang, Lei,et al. Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(8):2243-2260.
APA	Choudhary, Mani Kant,Nomura, Yuko,Wang, Lei,Nakagami, Hirofumi,&Somers, David E..(2015).Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways.MOLECULAR & CELLULAR PROTEOMICS,14(8),2243-2260.
MLA	Choudhary, Mani Kant,et al."Quantitative Circadian Phosphoproteomic Analysis of Arabidopsis Reveals Extensive Clock Control of Key Components in Physiological, Metabolic, and Signaling Pathways".MOLECULAR & CELLULAR PROTEOMICS 14.8(2015):2243-2260.

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