H+-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat

doi:10.1111/pce.12557

	H+-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat
	Lv, Sulian; Jiang, Ping; Nie, Lingling; Chen, Xianyang; Tai, Fang; Wang, Duoliya; Fan, Pengxiang; Feng, Juanjuan; Bao, Hexigeduleng; Wang, Jinhui; Li, Yinxin
	2015
发表期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
卷号	38 期号:11 页码:2433-2449
摘要	High salinity and nitrogen (N) deficiency in soil are two key factors limiting crop productivity, and they usually occur simultaneously. Here we firstly found that H+-PPase is involved in salt-stimulated NO3- uptake in the euhalophyte Salicornia europaea. Then, two genes (named SeVP1 and SeVP2) encoding H+-PPase from S.europaea were characterized. The expression of SeVP1 and SeVP2 was induced by salt stress and N starvation. Both SeVP1 or SeVP2 transgenic Arabidopsis and wheat plants outperformed the wild types (WTs) when high salt and low N occur simultaneously. The transgenic Arabidopsis plants maintained higher K+/Na+ ratio in leaves and exhibited increased NO3- uptake, inorganic pyrophosphate-dependent vacuolar nitrate efflux and assimilation capacity under this double stresses. Furthermore, they had more soluble sugars in shoots and roots and less starch accumulation in shoots than WT. These performances can be explained by the up-regulated expression of ion, nitrate and sugar transporter genes in transgenic plants. Taken together, our results suggest that up-regulation of H+-PPase favours the transport of photosynthates to root, which could promote root growth and integrate N and carbon metabolism in plant. This work provides potential strategies for improving crop yields challenged by increasing soil salinization and shrinking farmland. High salinity and nitrogen (N) deficiency which usually occur simultaneously in soil are two key factors limiting crop productivity. Here we firstly found that H+-pyrophosphatase (H+-PPase) is involved in salt stimulated NO3- uptake in the euhalophyte Salicornia europaea, and up-regulation of H+-pyrophosphatase confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat. This work provides alternative strategy for improving crop yields challenged by increasing soil salinization and shrinking farmland via H+-PPase regulation.
关键词	H+-PPase nitrogen metabolism Salicornia europaeaL salinity sugar transport
学科领域	Plant Sciences
DOI	10.1111/pce.12557
收录类别	SCI
语种	英语
WOS关键词	DEPENDENT NITRATE TRANSPORT ; ZOSTERA-MARINA L ; PLASMA-MEMBRANE ; HETEROLOGOUS EXPRESSION ; SIGNAL-TRANSDUCTION ; MINERAL-COMPOSITION ; REDUCTASE-ACTIVITY ; SALINITY ; PLANTS ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000363884600020
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
EISSN	1365-3040
资助机构	Chinese Ministry of Agriculture [2014ZX08009-003-002] ; National Natural Science Foundation of China [31270421]
作者邮箱	yxli@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25715
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Lv, Sulian,Jiang, Ping,Nie, Lingling,et al. H+-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat[J]. PLANT CELL AND ENVIRONMENT,2015,38(11):2433-2449.
APA	Lv, Sulian.,Jiang, Ping.,Nie, Lingling.,Chen, Xianyang.,Tai, Fang.,...&Li, Yinxin.(2015).H+-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat.PLANT CELL AND ENVIRONMENT,38(11),2433-2449.
MLA	Lv, Sulian,et al."H+-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat".PLANT CELL AND ENVIRONMENT 38.11(2015):2433-2449.

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