Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes

doi:10.1016/j.bbabio.2014.11.005

IB-CAS > 中科院光生物学重点实验室

	Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes
	Sun, Ruixue 1; Liu, Kun ; Dong, Lianqing 1; Wu, Yuling 1; Paulsen, Harald 2; Yang, Chunhong
	2015
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN	0005-2728
卷号	1847 期号:2 页码:248-261
摘要	Minor antennae of photosystem (PS) II, located between the PSII core complex and the major antenna (LHCII), are important components for the structural and functional integrity of PSII supercomplexes. In order to study the functional significance of minor antennae in the energetic coupling between LHCII and the PSII core, characteristics of PSII-LHCII proteoliposomes, with or without minor antennae, were investigated. Two types of PSII preparations containing different antenna compositions were isolated from pea: 1) the PSII preparation composed of the PSII core complex, all of the minor antennae, and a small amount of major antennae (MCC); and 2) the purified PSII dimeric core complexes without periphery antenna (CC). They were incorporated, together with LHCII, into liposomes composed of thylakoid membrane lipids. The spectroscopic and functional characteristics were measured. 77 K fluorescence emission spectra revealed an increased spectral weight of fluorescence from PSII reaction center in the CC-LHCII proteoliposomes, implying energetic coupling between LHCII and CC in the koteoliposomes lacking minor antennae. This result was further confirmed by chlorophyll a fluorescence induction kinetics. The incorporation of LHCII together with CC markedly increased the antenna cross-section of the PSII core complex. The 2,6-dichlorophenolindophenol photoreduction measurement implied that the lack of minor antennae in PSII supercomplexes did not block the energy transfer from LHCII to the PSII core complex. In conclusion, it is possible, in liposomes, that LHCII transfer energy directly to the PSII core complex, in the absence of minor antennae. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Proteoliposome Protein-protein interaction Photosystem II Light-harvesting complex Minor antenna
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbabio.2014.11.005
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEX ; CHLOROPHYLL-PROTEIN COMPLEXES ; TANDEM MASS-SPECTROMETRY ; SUPRAMOLECULAR ORGANIZATION ; CRYSTAL-STRUCTURE ; ARABIDOPSIS-THALIANA ; THYLAKOID MEMBRANES ; ANGSTROM RESOLUTION ; HIGHER-PLANTS ; CHLAMYDOMONAS-REINHARDTII
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000348337400012
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	0006-3002
资助机构	National Basic Research Program of China [2011CBA00904] ; Knowledge Innovation Program of the Chinese Academy of Sciences [KSZD-EW-Z-018] ; National Natural Science Foundation of China [31070212, 31370275] ; Deutsche Forschungsgemeinschaft [SFB 625-TP B7]
作者邮箱	yangch@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25718
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Nanxincun 20, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Johannes Gutenberg Univ Mainz, Inst Allgemeine Bot, D-55099 Mainz, Germany
推荐引用方式 GB/T 7714	Sun, Ruixue,Liu, Kun,Dong, Lianqing,et al. Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2015,1847(2):248-261.
APA	Sun, Ruixue,Liu, Kun,Dong, Lianqing,Wu, Yuling,Paulsen, Harald,&Yang, Chunhong.(2015).Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1847(2),248-261.
MLA	Sun, Ruixue,et al."Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1847.2(2015):248-261.

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