Plastidial Disproportionating Enzyme Participates in Starch Synthesis in Rice Endosperm by Transferring Maltooligosyl Groups from Amylose and Amylopectin to Amylopectin

doi:10.1104/pp.15.01411

	Plastidial Disproportionating Enzyme Participates in Starch Synthesis in Rice Endosperm by Transferring Maltooligosyl Groups from Amylose and Amylopectin to Amylopectin
	Dong, Xiangbai ; Zhang, Du ; Liu, Jie ; Liu, Qiao Quan 1; Liu, Hualiang ; Tian, Lihong ; Jiang, Ling 2; Qu, Le Qing
	2015
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	169 期号:4 页码:2496-2512
摘要	Plastidial disproportionating enzyme1 (DPE1), an alpha-1,4-D-glucanotransferase, has been thought to be involved in storage starch synthesis in cereal crops. However, the precise function of DPE1 remains to be established. We present here the functional identification of DPE1 in storage starch synthesis in rice (Oryza sativa) by endosperm-specific gene overexpression and suppression. DPE1 overexpression decreased amylose content and resulted in small and tightly packed starch granules, whereas DPE1 suppression increased amylose content and formed heterogeneous-sized, spherical, and loosely packed starch granules. Chains with degree of polymerization (DP) of 6 to 10 and 23 to 38 were increased, while chains with DP of 11 to 22 were decreased in amylopectin from DPE1-overexpressing seeds. By contrast, chains with DP of 6 to 8 and 16 to 36 were decreased, while chains with DP of 9 to 15 were increased in amylopectin from DPE1-suppressed seeds. Changes in DPE1 gene expression also resulted in modifications in the thermal and pasting features of endosperm starch granules. In vitro analyses revealed that recombinant DPE1 can break down amylose into maltooligosaccharides in the presence of Glc, while it can transfer maltooligosyl groups from maltooligosaccharide to amylopectin or transfer maltooligosyl groups within and among amylopectin molecules in the absence of Glc. Moreover, a metabolic flow of maltooligosyl groups from amylose to amylopectin was clearly identifiable when comparing DPE1-overexpressing lines with DPE1-suppressed lines. These findings demonstrate that DPE1 participates substantially in starch synthesis in rice endosperm by transferring maltooligosyl groups from amylose and amylopectin to amylopectin.
学科领域	Plant Sciences
DOI	10.1104/pp.15.01411
收录类别	SCI
语种	英语
WOS关键词	ALPHA-GLUCAN PHOSPHORYLASE ; PHYSICOCHEMICAL PROPERTIES ; CHAIN-LENGTH ; RHEOLOGICAL PROPERTIES ; POTATO ; WHEAT ; GELATINIZATION ; DEGRADATION ; ISOAMYLASE ; MUTATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000368472700012
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	Natural Science Foundation of China [31070226] ; National Program of Transgenic Variety Development of China [2014ZX08001-006]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25719
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Yangzhou Univ, Key Lab Plant Funct Genom, Yangzhou 225009, Jiangsu, Peoples R China 3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Dong, Xiangbai,Zhang, Du,Liu, Jie,et al. Plastidial Disproportionating Enzyme Participates in Starch Synthesis in Rice Endosperm by Transferring Maltooligosyl Groups from Amylose and Amylopectin to Amylopectin[J]. PLANT PHYSIOLOGY,2015,169(4):2496-2512.
APA	Dong, Xiangbai.,Zhang, Du.,Liu, Jie.,Liu, Qiao Quan.,Liu, Hualiang.,...&Qu, Le Qing.(2015).Plastidial Disproportionating Enzyme Participates in Starch Synthesis in Rice Endosperm by Transferring Maltooligosyl Groups from Amylose and Amylopectin to Amylopectin.PLANT PHYSIOLOGY,169(4),2496-2512.
MLA	Dong, Xiangbai,et al."Plastidial Disproportionating Enzyme Participates in Starch Synthesis in Rice Endosperm by Transferring Maltooligosyl Groups from Amylose and Amylopectin to Amylopectin".PLANT PHYSIOLOGY 169.4(2015):2496-2512.

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