Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size

doi:10.1093/jxb/eru415

	Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size
	Li, Zhichao 1; He, Chaoying
	2015
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	66 期号:1 页码:257-270
摘要	Physalis species show a significant variation in berry size; however, the underlying molecular basis is unknown. In this work, we showed that cell division difference in the ovaries might contribute to the ultimate berry size variation within Physalis species, and that mRNA abundance of Physalis floridana Cell Number Regulator1 (PfCNR1), the putative orthologue of the tomato fruit weight 2.2 (FW2.2), was negatively correlated with cell division in the ovaries. Moreover, heterochronic expression variation of the PfCNR1 genes in the ovaries concomitantly correlated with berry weight variation within Physalis species. In transgenic Physalis, multiple organ sizes could be negatively controlled by altering PfCNR1 levels, and cell division instead of cell expansion was primarily affected. PfCNR1 was shown to be anchored in the plasma membrane and to interact with PfAG2 (an AGAMOUS-like protein determining ovary identity). The expression of PfCYCD2;1, a putative orthologue of the mitosis-specific gene CyclinD2;1 in the cell cycle was negatively correlated with the PfCNR1 mRNA levels. PfAG2 was found to selectively bind to the CArG-box in the PfCYCD2;1 promoter and to repress PfCYCD2;1 expression, thus suggesting a PfAG2-mediated pathway for PfCNR1 to regulate cell division. The interaction of PfCNR1 with PfAG2 enhanced the repression of PfCYCD2;1 expression. The nuclear import of PfAG2 was essential in the proposed pathway. Our data provide new insights into the developmental pathways of a cell membrane-anchored protein that modulates cell division and governs organ size determination. This study also sheds light on the link between organ identity and organ growth in plants.
关键词	Berry size cell cycle fruit development natural variation Physalis seed
学科领域	Plant Sciences
DOI	10.1093/jxb/eru415
收录类别	SCI
语种	英语
WOS关键词	CASEIN KINASE-II ; TOMATO FRUIT ; ORGAN SIZE ; DOMESTICATION TRAITS ; PROTEIN INTERACTIONS ; REGULATORY CHANGE ; NATURAL VARIATION ; CHINESE LANTERN ; GENE FAMILY ; EVOLUTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000351660900021
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of China [30870175, 91331103] ; Hundred Talents Project of the Chinese Academy of Sciences
作者邮箱	chaoying@ibcas.ac.cn
作品OA属性	hybrid, Green Submitted, Green Published
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25725
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhichao,He, Chaoying. Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(1):257-270.
APA	Li, Zhichao,&He, Chaoying.(2015).Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size.JOURNAL OF EXPERIMENTAL BOTANY,66(1),257-270.
MLA	Li, Zhichao,et al."Physalis floridana Cell Number Regulator1 encodes a cell membrane-anchored modulator of cell cycle and negatively controls fruit size".JOURNAL OF EXPERIMENTAL BOTANY 66.1(2015):257-270.

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