Brassinosteroids are involved in Fe homeostasis in rice (Oryza sativa L.)

doi:10.1093/jxb/erv079

	Brassinosteroids are involved in Fe homeostasis in rice (Oryza sativa L.)
	Wang, Baolan ; Li, Gen ; Zhang, Wen-Hao
	2015
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	66 期号:9 页码:2749-2761
摘要	Brassinosteroids negatively regulate Fe transport and translocation in a strategy II rice plants.Brassinosteroids (BRs) are steroid hormones that modulate numerous physiological processes in plants. However, few studies have focused on the involvement of BRs in sensing and responding to the stress of mineral nutrient deficiency. In the present study, we evaluated the roles of BRs in the response of rice (Oryza sativa) to iron (Fe) deficiency during Fe uptake, transport, and translocation. Exogenous application of 24-epibrassinolide (EBR) to wild-type (WT) plants exaggerated leaf symptoms of Fe deficiency and suppressed growth. EBR increased and decreased Fe concentrations in roots and shoots, respectively, under both Fe-deficient and Fe-sufficient conditions. Transcripts involved in Fe homeostasis, including OsIRT1, OsYSL15, OsYSL2, OsNAS1, and OsNAS2, were enhanced by EBR under Fe-deficient conditions. EBR depressed expression of OsNAS1, OsNAS2, and OsYSL2 in shoots, and inhibited Fe transport and translocation via the phloem. Rice mutant d2-1, which is defective in BR biosynthesis, was more tolerant to Fe deficiency than the WT, and accumulated greater amounts of Fe in roots than the WT under Fe-sufficient conditions. A greater upregulation of OsIRT1, OsYSL15, OsYSL2, OsNAS1, and OsNAS2 in the d2-1 mutant compared to the WT was found under Fe-sufficient conditions, while expression of these genes in the d2-1 mutant was lower than in the WT under Fe-deficient conditions. The greater tolerance of the d2-1 mutant could be partly mitigated by exogenous application of EBR. These novel findings highlight the important role of BR in mediating the response of strategy II plants to Fe deficiency by regulating Fe uptake and translocation in rice.
关键词	Brassinosteroids (BRs) d2-1 mutant Fe deficiency Fe translocation Fe uptake rice (Oryza sativa) strategy II plant
学科领域	Plant Sciences
DOI	10.1093/jxb/erv079
收录类别	SCI
语种	英语
WOS关键词	LONG-DISTANCE TRANSPORT ; METAL-NICOTIANAMINE TRANSPORTER ; IRON-DEFICIENCY RESPONSES ; TRANSCRIPTION FACTOR ; ARABIDOPSIS-THALIANA ; CUCUMIS-SATIVUS ; PLANTS ; GENES ; TOLERANCE ; CITRATE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000354742200029
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Natural Science Foundation of China [31101594] ; State Key Laboratory of Vegetation and Environmental Change
作者邮箱	whzhang@ibcas.ac.cn
作品OA属性	Green Published, hybrid, Green Submitted
引用统计	被引频次：50[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25732
专题	植被与环境变化国家重点实验室
作者单位	1.[Wang, Baolan 2.Li, Gen 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Baolan,Li, Gen,Zhang, Wen-Hao. Brassinosteroids are involved in Fe homeostasis in rice (Oryza sativa L.)[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(9):2749-2761.
APA	Wang, Baolan,Li, Gen,&Zhang, Wen-Hao.(2015).Brassinosteroids are involved in Fe homeostasis in rice (Oryza sativa L.).JOURNAL OF EXPERIMENTAL BOTANY,66(9),2749-2761.
MLA	Wang, Baolan,et al."Brassinosteroids are involved in Fe homeostasis in rice (Oryza sativa L.)".JOURNAL OF EXPERIMENTAL BOTANY 66.9(2015):2749-2761.

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