Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates

doi:10.1093/molbev/msv156

	Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates
	Liu, Hai-Jing ; Tang, Zhen-Xin ; Han, Xue-Min ; Yang, Zhi-Ling ; Zhang, Fu-Min ; Yang, Hai-Ling ; Liu, Yan-Jing ; Zeng, Qing-Yin
	2015
发表期刊	MOLECULAR BIOLOGY AND EVOLUTION
ISSN	0737-4038
卷号	32 期号:11 页码:2844-2859
摘要	Whole-genome duplication (WGD), or polyploidy, is a major force in plant genome evolution. A duplicate of all genes is present in the genome immediately following a WGD event. However, the evolutionary mechanisms responsible for the loss of, or retention and subsequent functional divergence of polyploidy-derived duplicates remain largely unknown. In this study we reconstructed the evolutionary history of the glutathione S-transferase (GST) gene family from the soybean genome, and identified 72 GST duplicated gene pairs formed by a recent Glycine-specific WGD event occurring approximately 13 Ma. We found that 72% of duplicated GST gene pairs experienced gene losses or pseudogenization, whereas 28% of GST gene pairs have been retained in the soybean genome. The GST pseudogenes were under relaxed selective constraints, whereas functional GSTs were subject to strong purifying selection. Plant GST genes play important roles in stress tolerance and detoxification metabolism. By examining the gene expression responses to abiotic stresses and enzymatic properties of the ancestral and current proteins, we found that polyploidy-derived GST duplicates show the divergence in enzymatic activities. Through site-directed mutagenesis of ancestral proteins, this study revealed that nonsynonymous substitutions of key amino acid sites play an important role in the divergence of enzymatic functions of polyploidy-derived GST duplicates. These findings provide new insights into the evolutionary and functional dynamics of polyploidy-derived duplicate genes.
关键词	gene and genome duplication gene family glutathione S-transferase enzyme activity functional divergence
学科领域	Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
DOI	10.1093/molbev/msv156
收录类别	SCI
语种	英语
WOS关键词	FUNCTIONAL DIVERGENCE ; GLUTATHIONE TRANSFERASES ; GENE FAMILY ; POLYPLOIDY ; CONSEQUENCES ; ACID ; TRIPLICATION ; EXPRESSION ; SEQUENCE ; EVENTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000363033100004
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1537-1719
资助机构	National Science Foundation of China [91231103, 31425006] ; Chinese Academy of Sciences [KSCX2-EW-J-1]
作者邮箱	yanjing.liu@ibcas.ac.cn ; qingyin.zeng@ibcas.ac.cn
作品OA属性	Green Published, hybrid, Green Submitted
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25740
专题	系统与进化植物学国家重点实验室
作者单位	1.[Liu, Hai-Jing 2.Zhang, Fu-Min 3.Liu, Yan-Jing 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.[Tang, Zhen-Xin 7.Han, Xue-Min 8.Yang, Zhi-Ling 9.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hai-Jing,Tang, Zhen-Xin,Han, Xue-Min,et al. Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates[J]. MOLECULAR BIOLOGY AND EVOLUTION,2015,32(11):2844-2859.
APA	Liu, Hai-Jing.,Tang, Zhen-Xin.,Han, Xue-Min.,Yang, Zhi-Ling.,Zhang, Fu-Min.,...&Zeng, Qing-Yin.(2015).Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates.MOLECULAR BIOLOGY AND EVOLUTION,32(11),2844-2859.
MLA	Liu, Hai-Jing,et al."Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates".MOLECULAR BIOLOGY AND EVOLUTION 32.11(2015):2844-2859.

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