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| Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis | |
Yang, Zhipan; Shang, Zengzhen1; Wang, Lei1 ; Lu, Qingtao; Wen, Xiaogang; Chi, Wei; Zhang, Lixin; Lu, Congming
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| 2015 | |
| Source Publication | PHOTOSYNTHESIS RESEARCH
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| ISSN | 0166-8595 |
| Volume | 126Issue:2-3Pages:285-300 |
| Abstract | To investigate the molecular mechanism of chloroplast biogenesis and development, we characterized an Arabidopsis mutant (dg169, delayed greening 169) which showed growth retardation and delayed greening phenotype in leaves. Newly emerged chlorotic leaves recovered gradually with leaf development in the mutant, and the mature leaves showed similar phenotype to those of wild-typewild-type plants. Compared with wild-type, the chloroplasts were oval-shaped and smaller and the thylakoid membranes were less abundant in yellow section of young leaves of dg169. In addition, the functions of photosystem II (PSII) and photosystem I (PSI) were also impaired. Furthermore, the amount of core subunits of PSII and PSI, as well as PSII and PSI complexes reduced in yellow section of young leaves of dg169. Map-based positional cloning identified that phenotype of dg169 was attributed to a point mutation of ATase2 which converts the conserved Ile-155 residue to Asn. ATase2 catalyzes the first step of de novo purine biosynthesis. This mutation resulted in impaired purine synthesis and a significant decrease in ATP, ADP, GTP and GDP contents. The analysis of ATase2-GFP protein fusion showed that ATase2 was localized to nucleoid of chloroplasts. Our results further demonstrated that the levels of PEP-dependent transcripts in yellow section of young leaves of dg169 were decreased while NEP-dependent and both PEP- and NEP-dependent transcripts and chloroplast DNA replications were increased. The results in this study suggest that ATase2 plays an essential role in early chloroplast development through maintaining PEP function. |
| Keyword | Arabidopsis ATase2 Chloroplast development Chloroplast gene expression Purine biosynthesis |
| Subject Area | Plant Sciences |
| DOI | 10.1007/s11120-015-0131-z |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ACTIVE CHROMOSOME COMPLEX ; RNA-POLYMERASE ; PLASTID CHROMOSOME ; LEAF VARIEGATION ; PHOTOSYSTEM-II ; MESSENGER-RNA ; POTATO-TUBERS ; SIGMA FACTORS ; THALIANA ; PROTEIN |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000362006900009 |
| Publisher | SPRINGER |
| Special Issue | SI |
| Subtype | Article |
| Publication Place | DORDRECHT |
| EISSN | 1573-5079 |
| Funding Organization | State Key Basic Research and Development Plan of China [2015CB150105] ; Chinese Academy of Sciences [KGZD-EW-T05] |
| Corresponding Author Email | yangzp@ibcas.ac.cn ; lucm@ibcas.ac.cn |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25743 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Zhipan,Shang, Zengzhen,Wang, Lei,et al. Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis[J]. PHOTOSYNTHESIS RESEARCH,2015,126(2-3):285-300. |
| APA | Yang, Zhipan.,Shang, Zengzhen.,Wang, Lei.,Lu, Qingtao.,Wen, Xiaogang.,...&Lu, Congming.(2015).Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis.PHOTOSYNTHESIS RESEARCH,126(2-3),285-300. |
| MLA | Yang, Zhipan,et al."Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis".PHOTOSYNTHESIS RESEARCH 126.2-3(2015):285-300. |
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| Yang-2015-Purine bio（5353KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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