REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis

doi:10.1007/s11120-015-0146-5

IB-CAS > 中科院光生物学重点实验室

	REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis
	Xu, Gang 1; Guo, Haiyan 1; Zhang, Dong 1; Chen, Dongqin ; Jiang, Zhimin 1; Lin, Rongcheng
	2015
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	126 期号:2-3 页码:331-340
摘要	Chlorophyll biosynthesis plays a crucial role in the greening process and survival of etiolated seedlings and yet the mechanism underlying the regulation of this process is poorly understood. Upon light stimulation, NADPH: protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide. Whereas this represents a key step in the chlorophyll biosynthetic pathway, the regulation of POR remains largely unknown. Three POR isoforms exist in Arabidopsis thaliana, i.e., PORA, PORB, and PORC. In this study, we identified a transcription factor, REVEILLE1 (RVE1), that binds directly to the PORA promoter through the EE-box cis-regulatory element. Analysis of PORA expression in RVE1 loss-of-function (rve1) and overexpression (RVE1-OX) Arabidopsis plants showed that RVE1 positively regulates the transcription of PORA. We found that Pchlide levels were reduced in RVE1-OX seedlings. Furthermore, rve1 etiolated seedlings had lower greening rates than the wild type when exposed to light, whereas RVE1-OX seedlings had higher greening rates. In addition, when etiolated seedlings were exposed to light, RVE1-OX plants had less reactive oxygen species (ROS) accumulation and cell death than the wild type, and had reduced levels of ROS-responsive gene expression. Taken together, our study reveals an important role for RVE1 in regulating chlorophyll biosynthesis and promoting seedling greening during early plant growth and development.
关键词	Chlorophyll biosynthesis RVE1 POR Seedling greening
学科领域	Plant Sciences
DOI	10.1007/s11120-015-0146-5
收录类别	SCI
语种	英语
WOS关键词	CHLOROPHYLL BIOSYNTHESIS ; REGULATE CHLOROPHYLL ; STRESS RESPONSES ; GENE-EXPRESSION ; SINGLET OXYGEN ; LIGHT ; POR ; THALIANA ; PROTEIN ; DEETIOLATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000362006900013
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Natural Science Foundation of China [31170221, 31325002] ; Ministry of Agriculture of China [2014ZX08009-003]
作者邮箱	rclin@ibcas.ac.cn
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25767
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Gang,Guo, Haiyan,Zhang, Dong,et al. REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis[J]. PHOTOSYNTHESIS RESEARCH,2015,126(2-3):331-340.
APA	Xu, Gang,Guo, Haiyan,Zhang, Dong,Chen, Dongqin,Jiang, Zhimin,&Lin, Rongcheng.(2015).REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis.PHOTOSYNTHESIS RESEARCH,126(2-3),331-340.
MLA	Xu, Gang,et al."REVEILLE1 promotes NADPH: protochlorophyllide oxidoreductase A expression and seedling greening in Arabidopsis".PHOTOSYNTHESIS RESEARCH 126.2-3(2015):331-340.

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