Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

	Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant
	Guan, Z-J ; Lu, S-B ; Huo, Y-L ; Guo, B.; Guan, Z-P ; Wei, Y-H
	2015
发表期刊	PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
ISSN	1851-5657
卷号	84 期号:2 页码:351-357
摘要	Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until 42 days from culturing and then decreased sharply. Malondialdehyde (MDA) of the mutant showed a significant decreasing trend after 21 days from culturing. Growth rate of the mutant was at times lower than that of the control during its callus differentiation, and the soluble protein content of the mutant callus decreased from explant cultivation until the 28th day of culture. The mutant had greater values of chlorophyll a, carotenoid and Chlorophyll contents than the control after 14 days of culturing, and Chlorophyll b content of the mutant showed a declining trend. The electrical conductivity trend of the mutant was consistent with that in the control. It indicated that in terms of the organogenesis or somatic embryogenesis pattern, protein synthesis and catabolism were very active, and a number of antioxidant enzyme activities were consistent in the early development stages of the two regeneration systems. These findings were useful for the regeneration of the mutant.
关键词	Physiological Biochemical Organogenesis Somatic embryogenesis HBsAg-transgenic Cherry tomato Mutant
学科领域	Plant Sciences
收录类别	SCI
语种	英语
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000369315000014
出版者	TECH SCIENCE PRESS
文献子类	Article
出版地	HENDERSON
资助机构	National Natural Science Foundation of China [31200422, 31360136, 31560168] ; China's Postdoctoral Science Foundation [2012M520455, 2013T60193] ; Yuncheng University Doctor Scientific research project [YQ-2014022] ; Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education [PK2014009] ; Jiangxi Normal University, Jiangxi Province Natural Science Foundation of China [20151BAB204007]
作者邮箱	zhengjunguan@126.com ; weiyahui@nwu.edu.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25777
专题	植被与环境变化国家重点实验室
作者单位	1.Yuncheng Univ, Dept Life Sci, Yuncheng 044000, Shanxi, Peoples R China 2.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Jiangxi, Peoples R China 3.[Guan, Z-J 4.Guo, B. 5.Northwest Univ, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Shannxi, Peoples R China 6.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 7.Yuncheng Univ, Ctr Biol & Chem Expt, Yuncheng 044000, Shanxi, Peoples R China 8.Shanxi Normal Univ, Coll Food Sci, Linfen 04100, Peoples R China
推荐引用方式 GB/T 7714	Guan, Z-J,Lu, S-B,Huo, Y-L,et al. Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant[J]. PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY,2015,84(2):351-357.
APA	Guan, Z-J,Lu, S-B,Huo, Y-L,Guo, B.,Guan, Z-P,&Wei, Y-H.(2015).Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant.PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY,84(2),351-357.
MLA	Guan, Z-J,et al."Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant".PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY 84.2(2015):351-357.

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