Biochemical and structural study of Arabidopsis hexokinase 1

doi:10.1107/S1399004714026091

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	Biochemical and structural study of Arabidopsis hexokinase 1
	Feng, Juan 1; Zhao, Shun 1; Chen, Xuemin 1; Wang, Wenda ; Dong, Wei 1; Chen, Jinghua 1; Shen, Jian-Ren; Liu, Lin ; Kuang, Tingyun
	2015
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN	1399-0047
卷号	71 页码:367-375
摘要	Hexokinase 1 from Arabidopsis thaliana (AtHXK1) plays a dual role in glycolysis and sugar sensing for vital metabolic and physiological processes. The uncoupling of glucose signalling from glucose metabolism was demonstrated by the analysis of two mutants (AtHXK1(G104D) and AtHXK1(S177A)) that are catalytically inactive but still functional in signalling. In this study, substrate-binding experiments indicate that the two catalytically inactive mutants have a high affinity for glucose, and an ordered substrate-binding mechanism has been observed for wild-type AtHXK1. The structure of AtHXK1 was determined both in its inactive unliganded form and in its active glucose-bound form at resolutions of 1.8 and 2.0 angstrom, respectively. These structures reveal a domain rearrangement of AtHXK1 upon glucose binding. The 2.1 angstrom resolution structure of AtHXK1(S177A) in the glucose-bound form shows similar glucose-binding interactions as the wild type. A glucose-sensing network has been proposed based on these structures. Taken together, the results provide a structural explanation for the dual functions of AtHXK1.
学科领域	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
DOI	10.1107/S1399004714026091
收录类别	SCI
语种	英语
WOS关键词	SKELETAL-MUSCLE HEXOKINASE ; X-RAY CRYSTALLOGRAPHY ; YEAST-HEXOKINASE ; CRYSTAL-STRUCTURE ; ALLOSTERIC REGULATION ; ISOTOPE-EXCHANGE ; MOLECULAR-BASIS ; GLUCOSE ; GLUCOKINASE ; COMPLEX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000349439900020
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
资助机构	National Basic Research Program of China [2011CBA00901] ; National Natural Science Foundation of China [31170688] ; Chinese Academy of Sciences
作者邮箱	liulin@ibcas.ac.cn ; kuangty@ibcas.ac.cn
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25785
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Feng, Juan,Zhao, Shun,Chen, Xuemin,et al. Biochemical and structural study of Arabidopsis hexokinase 1[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2015,71:367-375.
APA	Feng, Juan.,Zhao, Shun.,Chen, Xuemin.,Wang, Wenda.,Dong, Wei.,...&Kuang, Tingyun.(2015).Biochemical and structural study of Arabidopsis hexokinase 1.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,71,367-375.
MLA	Feng, Juan,et al."Biochemical and structural study of Arabidopsis hexokinase 1".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 71(2015):367-375.

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