Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation

doi:10.1093/jxb/erv173

	Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation
	Zhu, Zhixin 1; Wang, Hailong 1; Wang, Yiting 1; Guan, Shan ; Wang, Fang 1; Tang, Jingyu 1; Zhang, Ruijuan 1; Xie, Lulu 1; Lu, Yingqing1
	2015
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	66 期号:13 页码:3775-3789
摘要	Cellular activities such as compound synthesis often require the transcriptional activation of an entire pathway; however, the molecular mechanisms underlying pathway activation have rarely been explained. Here, the cis regulatory architecture of the anthocyanin pathway genes targeted by the transcription factor (TF) complex including MYB, bHLH, and WDR was systematically analysed in one species and the findings extended to others. In Ipomoea purpurea, the IpMYB1-IpbHLH2-IpWDR1 (IpMBW) complex was found to be orthologous to the PAP1-GL3-TTG1 (AtPGT) complex of Arabidopsis thaliana, and interacted with a 7-bp MYB-recognizing element (MRE) and a 6-bp bHLH-recognizing element (BRE) at the proximal promoter region of the pathway genes. There was little transcription of the gene in the absence of the MRE or BRE. The cis elements identified experimentally converged on two syntaxes, ANCNNCC for MREs and CACN(A/C/T)(G/T) for BREs, and our bioinformatic analysis showed that these were present within anthocyanin gene promoters in at least 35 species, including both gymnosperms and angiosperms. For the anthocyanin pathway, IpMBW and AtPGT recognized the interspecific promoters of both early and later genes. In A. thaliana, the seed-specific TF complex (TT2, TT8, and TTG1) may regulate all the anthocyanin pathway genes, in addition to the proanthocyanidin-specific BAN. When multiple TF complexes in the anthocyanin pathway were compared, the cis architecture played a role larger than the TF complex in determining the variation in promoter activity. Collectively, a cis logic common to the pathway gene promoters was found, and this logic is essential for the trans factors to regulate the pathway.
关键词	Anthocyanin pathway Arabidopsis bHLH cis element Ipomoea MBW complex MYB promoter activity promoter architecture regulatory module trans factor transcription initiation WDR
学科领域	Plant Sciences
DOI	10.1093/jxb/erv173
收录类别	SCI
语种	英语
WOS关键词	CHALCONE SYNTHASE PROMOTER ; ARABIDOPSIS-THALIANA ; MORNING GLORY ; TRANSCRIPTION FACTORS ; REPEAT PROTEIN ; MYB DOMAIN ; PHENYLPROPANOID BIOSYNTHESIS ; EXPRESSION PATTERNS ; FLAVONOID SYNTHESIS ; IPOMOEA-PURPUREA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000358533400010
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Science Foundation of China [91331116, 31070263] ; Chinese Academy of Sciences [KSCX2-YW-N-043]
作者邮箱	yqlu@ibcas.ac.cn
作品OA属性	hybrid, Green Published, Green Submitted
引用统计	被引频次：72[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25790
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Zhixin,Wang, Hailong,Wang, Yiting,et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(13):3775-3789.
APA	Zhu, Zhixin.,Wang, Hailong.,Wang, Yiting.,Guan, Shan.,Wang, Fang.,...&Lu, Yingqing.(2015).Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.JOURNAL OF EXPERIMENTAL BOTANY,66(13),3775-3789.
MLA	Zhu, Zhixin,et al."Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation".JOURNAL OF EXPERIMENTAL BOTANY 66.13(2015):3775-3789.

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