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Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation | |
Zhu, Zhixin1; Wang, Hailong1; Wang, Yiting1; Guan, Shan; Wang, Fang1; Tang, Jingyu1; Zhang, Ruijuan1; Xie, Lulu1; Lu, Yingqing1 | |
2015 | |
发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY |
ISSN | 0022-0957 |
卷号 | 66期号:13页码:3775-3789 |
摘要 | Cellular activities such as compound synthesis often require the transcriptional activation of an entire pathway; however, the molecular mechanisms underlying pathway activation have rarely been explained. Here, the cis regulatory architecture of the anthocyanin pathway genes targeted by the transcription factor (TF) complex including MYB, bHLH, and WDR was systematically analysed in one species and the findings extended to others. In Ipomoea purpurea, the IpMYB1-IpbHLH2-IpWDR1 (IpMBW) complex was found to be orthologous to the PAP1-GL3-TTG1 (AtPGT) complex of Arabidopsis thaliana, and interacted with a 7-bp MYB-recognizing element (MRE) and a 6-bp bHLH-recognizing element (BRE) at the proximal promoter region of the pathway genes. There was little transcription of the gene in the absence of the MRE or BRE. The cis elements identified experimentally converged on two syntaxes, ANCNNCC for MREs and CACN(A/C/T)(G/T) for BREs, and our bioinformatic analysis showed that these were present within anthocyanin gene promoters in at least 35 species, including both gymnosperms and angiosperms. For the anthocyanin pathway, IpMBW and AtPGT recognized the interspecific promoters of both early and later genes. In A. thaliana, the seed-specific TF complex (TT2, TT8, and TTG1) may regulate all the anthocyanin pathway genes, in addition to the proanthocyanidin-specific BAN. When multiple TF complexes in the anthocyanin pathway were compared, the cis architecture played a role larger than the TF complex in determining the variation in promoter activity. Collectively, a cis logic common to the pathway gene promoters was found, and this logic is essential for the trans factors to regulate the pathway. |
关键词 | Anthocyanin pathway Arabidopsis bHLH cis element Ipomoea MBW complex MYB promoter activity promoter architecture regulatory module trans factor transcription initiation WDR |
学科领域 | Plant Sciences |
DOI | 10.1093/jxb/erv173 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | CHALCONE SYNTHASE PROMOTER ; ARABIDOPSIS-THALIANA ; MORNING GLORY ; TRANSCRIPTION FACTORS ; REPEAT PROTEIN ; MYB DOMAIN ; PHENYLPROPANOID BIOSYNTHESIS ; EXPRESSION PATTERNS ; FLAVONOID SYNTHESIS ; IPOMOEA-PURPUREA |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000358533400010 |
出版者 | OXFORD UNIV PRESS |
文献子类 | Article |
出版地 | OXFORD |
EISSN | 1460-2431 |
资助机构 | National Science Foundation of China [91331116, 31070263] ; Chinese Academy of Sciences [KSCX2-YW-N-043] |
作者邮箱 | yqlu@ibcas.ac.cn |
作品OA属性 | hybrid, Green Published, Green Submitted |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25790 |
专题 | 系统与进化植物学国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Zhixin,Wang, Hailong,Wang, Yiting,et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(13):3775-3789. |
APA | Zhu, Zhixin.,Wang, Hailong.,Wang, Yiting.,Guan, Shan.,Wang, Fang.,...&Lu, Yingqing.(2015).Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.JOURNAL OF EXPERIMENTAL BOTANY,66(13),3775-3789. |
MLA | Zhu, Zhixin,et al."Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation".JOURNAL OF EXPERIMENTAL BOTANY 66.13(2015):3775-3789. |
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