Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability

doi:10.1016/j.bbrc.2015.03.043

IB-CAS > 中科院光生物学重点实验室

	Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability
	Li, Li 1,2; Deng, Dandan 2; Chen, Xi 2; Wu, Baomei 2; Hu, Ke 2; Qiu, Tianhang 2; Cui, Suxia 2; Huang, Fang
	2015
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	460 期号:2 页码:386-391
摘要	Green vegetative tissues of the moss Physcomitrella patens possess a powerful ability to tolerate severe drought stress. Proteomics analysis have revealed that a large number of late embryogenesis abundant (LEA) proteins were key players in the drought tolerance of the photosynthetic tissues. PpLEA4-20, a member of the moss LEA protein family, was selected for further function study using an ectopic expression method in rice. Through molecular identification via PCR, southern blotting and TAIL-PCR, we demonstrated that the PpLEA4-20 gene was transformed and inserted into a non-encoded region in chromosome 4 of rice and expressed stably in transgenic rice. Unexpectedly, PpLEA4-20 protein emerged as two high-expressed spots on 2-D gels generated from transgenic rice, suggesting that PpLEA4-20 proteins are complete compatible and might be modified in rice. Both growth and physiological analysis showed that seedlings of transgenic PpLEA4-20 rice displayed altered phenotypes and tolerance to salt. In addition, electrolyte leakage was reduced in transgenic PpLEA4-20 compared to wild type under stress conditions. Anti-aggregation analysis found that the PpLEA4-20 protein expressed in rice remained soluble at high temperature and in addition to some native proteins from transgenic PpLEA4-20 rice. Based on Nano LC MS/MS analysis, we identified several proteins from transgenic PpLEA4-20 rice of increased heat-stability. Our results provide evidence for a role of PpLEA4-20 in salt tolerance and stabilization of client proteins. (C) 2015 Elsevier Inc. All rights reserved.
关键词	PpLEA4-20 Transformation Rice 2-DE Membrane protection Protein targets
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbrc.2015.03.043
收录类别	SCI
语种	英语
WOS关键词	EMBRYOGENESIS ABUNDANT PROTEINS ; PHYSCOMITRELLA-PATENS ; ARABIDOPSIS-THALIANA ; FREEZING TOLERANCE ; LEA PROTEINS ; WATER-STRESS ; PLANTS ; DESICCATION ; DEHYDRATION ; RESPONSES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000354230300042
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
文献子类	Article
出版地	SAN DIEGO
EISSN	1090-2104
资助机构	National Nature Science Foundation of China [31370816] ; Major Scientific Research Projects of the Ministry of Agriculture of China [2011ZX08001-003]
作者邮箱	sxcui@cnu.edu.cn ; fhuang@ibcas.ac.cn
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25792
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Li, Li,Deng, Dandan,Chen, Xi,et al. Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,460(2):386-391.
APA	Li, Li.,Deng, Dandan.,Chen, Xi.,Wu, Baomei.,Hu, Ke.,...&Huang, Fang.(2015).Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,460(2),386-391.
MLA	Li, Li,et al."Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 460.2(2015):386-391.

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