Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)

doi:10.1007/s11103-015-0331-0

	Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)
	Yan, Shuo 1; Wang, Zhongni 1; Liu, Yuan ; Li, Wei 2; Wu, Feng ; Lin, Xuelei ; Meng, Zheng
	2015
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	88 期号:4-5 页码:415-428
摘要	Late stage pollen-specific promoters are important tools in crop molecular breeding. Several such promoters, and their functional motifs, have been well characterized in dicotyledonous plants such as tomato and tobacco. However, knowledge about the functional architecture of such promoters is limited in the monocotyledonous plant rice. Here, pollen-late-stage-promoter 1 (PLP1) and pollen-late-stage-promoter 2 (PLP2) were characterized using a stable transformation system in rice. Histochemical staining showed that the two promoters exclusively drive GUS expression in late-stage pollen grains in rice. 5' deletion analysis revealed that four regions, including the -1159 to -720 and the -352 to -156 regions of PLP1 and the -740 to -557 and the -557 to -339 regions of PLP2, are important in maintaining the activity and specificity of these promoters. Motif mutation analysis indicated that 'AGAAA' and 'CAAT' motifs in the -740 to -557 region of PLP2 act as enhancers in the promoter. Gain of function experiments indicated that the novel TA-rich motif 'TACATAA' and 'TATTCAT' in the core region of the PLP1 and PLP2 promoters is necessary, but not sufficient, for pollen-specific expression in rice. Our results provide evidence that the enhancer motif 'AGAAA' is conserved in the pollen-specific promoters of both monocots and eudicots, but that some functional architecture characteristics are different.
关键词	Pollen-specific promoter Rice cis-Element Functional architecture Stable transformation
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-015-0331-0
收录类别	SCI
语种	英语
WOS关键词	TISSUE-SPECIFIC EXPRESSION ; CIS-REGULATORY ELEMENTS ; MALE-STERILITY ; TRANSGENIC RICE ; GENE-EXPRESSION ; MESSENGER-RNAS ; PLANTS ; ARABIDOPSIS ; ANTHER ; TOBACCO
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000357052100008
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National Natural Science Foundation of China [31270280] ; Ministry of Science and Technology of China [2011CB100405]
作者邮箱	zhmeng@ibcas.ac.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25802
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 3.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China
推荐引用方式 GB/T 7714	Yan, Shuo,Wang, Zhongni,Liu, Yuan,et al. Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)[J]. PLANT MOLECULAR BIOLOGY,2015,88(4-5):415-428.
APA	Yan, Shuo.,Wang, Zhongni.,Liu, Yuan.,Li, Wei.,Wu, Feng.,...&Meng, Zheng.(2015).Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.).PLANT MOLECULAR BIOLOGY,88(4-5),415-428.
MLA	Yan, Shuo,et al."Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.)".PLANT MOLECULAR BIOLOGY 88.4-5(2015):415-428.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Yan-2015-Functional （1405KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文