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| Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea | |
Nie, Lingling; Feng, Juanjuan; Fan, Pengxiang1; Chen, Xianyang; Guo, Jie; Lv, Sulian; Bao, Hexigeduleng2; Jia, Weitao; Tai, Fang; Jiang, Ping; Wang, Jinhui; Li, Yinxin
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| 2015 | |
| Source Publication | JOURNAL OF EXPERIMENTAL BOTANY
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| ISSN | 0022-0957 |
| Volume | 66Issue:15Pages:4497-4510 |
| Abstract | Improving crop nitrogen (N) use efficiency under salinity is essential for the development of sustainable agriculture in marginal lands. Salicornia europaea is a succulent euhalophyte that can survive under high salinity and N-deficient habitat conditions, implying that a special N assimilation mechanism may exist in this plant. In this study, phenotypic and physiological changes of S. europaea were investigated under different nitrate and NaCl levels. The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea. In addition, the shoot nitrate concentration and nitrate uptake rate of S. europaea were increased by NaCl treatment under both low N and high N conditions, suggesting that nitrate uptake in S. europaea was NaCl facilitated. Comparative proteomic analysis of root plasma membrane (PM) proteins revealed 81 proteins, whose abundance changed significantly in response to NaCl and nitrate. These proteins are involved in metabolism, cell signalling, transport, protein folding, membrane trafficking, and cell structure. Among them, eight proteins were calcium signalling components, and the accumulation of seven of the above-mentioned proteins was significantly elevated by NaCl treatment. Furthermore, cytosolic Ca2+ concentration ([Ca2+](cyt)) was significantly elevated in S. europaea under NaCl treatment. The application of the Ca2+ channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates. Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca2+ signalling was proposed. |
| Keyword | [Ca2+](cyt) calcium signalling 2D-DIGE NaCl nitrate uptake plasma membrane Salicornia europaea |
| Subject Area | Plant Sciences |
| DOI | 10.1093/jxb/erv216 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | STRESS-RESPONSIVE PROTEINS ; ARABIDOPSIS-THALIANA ; CYTOSOLIC CALCIUM ; NITROGEN NUTRITION ; SALT TOLERANCE ; SODIUM ; SALINITY ; PLANTS ; GROWTH ; TRANSPORT |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000359687400006 |
| Publisher | OXFORD UNIV PRESS |
| Subtype | Article |
| Publication Place | OXFORD |
| EISSN | 1460-2431 |
| Funding Organization | Chinese Ministry of Agriculture [2014ZX08009-003-002] ; National Natural Science Foundation of China [31200201] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-EW-STS-061-4] |
| Corresponding Author Email | yxli@ibcas.ac.cn |
| OA | Green Published, hybrid, Green Submitted |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/25810 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol PSC, Shanghai 201602, Peoples R China |
| Recommended Citation GB/T 7714 | Nie, Lingling,Feng, Juanjuan,Fan, Pengxiang,et al. Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(15):4497-4510. |
| APA | Nie, Lingling.,Feng, Juanjuan.,Fan, Pengxiang.,Chen, Xianyang.,Guo, Jie.,...&Li, Yinxin.(2015).Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.JOURNAL OF EXPERIMENTAL BOTANY,66(15),4497-4510. |
| MLA | Nie, Lingling,et al."Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea".JOURNAL OF EXPERIMENTAL BOTANY 66.15(2015):4497-4510. |
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| Nie-2015-Comparative（5173KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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