Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses

doi:10.1111/jipb.12241

IB-CAS > 中科院光生物学重点实验室

	Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses
	Jia, Xiao-Hui ; Zhang, Peng-Peng ; Shi, Ding-Ji ; Mi, Hua-Ling ; Zhu, Jia-Cheng ; Huang, Xi-Wen ; He, Pei-Min
	2015
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	57 期号:5 页码:468-476
摘要	Since pepc gene encoding phosphoenolpyruvate carboxylase (PEPCase) has been cloned from Anabaena sp. PCC 7120 and other cyanobacteria, the effects of pepc gene expression on photosynthesis have not been reported yet. In this study, we constructed mutants containing either upregulated (forward) or downregulated (reverse) pepc gene in Anabaena sp. PCC 7120. Results from real-time quantitative polymerase chain reaction (RT-qPCR), Western blot and enzymatic analysis showed that PEPCase activity was significantly reduced in the reverse mutant compared with the wild type, and that of the forward mutant was obviously increased. Interestingly, the net photosynthesis in both the reverse mutant and the forward mutant were higher than that of the wild type, but dark respiration was decreased only in the reverse mutant. The absorbance changes of P700 upon saturation pulse showed the photosystem I (PSI) activity was inhibited, as reflected by Y(I), and Y(NA) was elevated, and dark reduction of P700(+) was stimulated, indicating enhanced cyclic electron flow (CEF) around PSI in the reverse mutant. Additionally, the reverse mutant photosynthesis was higher than that of the wild type in low temperature, low and high pH, and high salinity, and this implies increased tolerance in the reverse mutant through downregulated pepc gene.
关键词	Anabaena sp PCC 7120 cyclic electron flow pepc gene photosynthesis tolerance
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/jipb.12241
收录类别	SCI
语种	英语
WOS关键词	PYRIDINE-NUCLEOTIDE DEHYDROGENASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; INTERSYSTEM CHAIN ; CHLAMYDOMONAS-REINHARDTII ; NAD(P)H DEHYDROGENASE ; STATE TRANSITIONS ; TRANSPORT ROUTES ; REDOX STATE ; CYANOBACTERIUM ; PHOTOINHIBITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000353493600002
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	National High-tech R&D Program (863 Program) [2009AA064401, 2014AA093506] ; Chinese Ministry of Environmental Protection [04] ; Shanghai Universities First-class Disciplines Project of Fisheries
作者邮箱	cyanoshi@163.com ; pmhe@shou.edu.cn
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25816
专题	中科院光生物学重点实验室
作者单位	1.[Jia, Xiao-Hui 2.Shi, Ding-Ji 3.Zhu, Jia-Cheng 4.Huang, Xi-Wen 5.Shanghai Ocean Univ, Minist Educ Shanghai, Key Lab Explorat & Utilizat Aquat Genet Resource, Shanghai 201306, Peoples R China 6.Louisiana State Univ, Dep Biol Sci, Baton Rouge, LA 70803 USA 7.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 8.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Jia, Xiao-Hui,Zhang, Peng-Peng,Shi, Ding-Ji,et al. Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2015,57(5):468-476.
APA	Jia, Xiao-Hui.,Zhang, Peng-Peng.,Shi, Ding-Ji.,Mi, Hua-Ling.,Zhu, Jia-Cheng.,...&He, Pei-Min.(2015).Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,57(5),468-476.
MLA	Jia, Xiao-Hui,et al."Regulation of pepc gene expression in Anabaena sp PCC 7120 and its effects on cyclic electron flow around photosystem I and tolerances to environmental stresses".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 57.5(2015):468-476.

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