Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells

doi:10.1007/s12217-015-9445-x

	Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells
	Zheng, Zhongyu 1; Zou, Junjie ; Li, Hanhai 1; Xue, Shan ; Le, Jie; Wang, Yuren 1
	2015
发表期刊	MICROGRAVITY SCIENCE AND TECHNOLOGY
ISSN	0938-0108
卷号	27 期号:6 页码:485-493
摘要	Gravitropism in plants is one of the most controversial issues. In the most wildly accepted starch-statolith hypothesis the sedimentation movement of amyloplasts in the gravisensing columella cells primarily triggers the asymmetric distribution of auxin which leads to the differential growth of the plant root. It has been gradually recognized that the inhomogeneous structures in statocytes arising from intracellular components such as cytoskeletons significantly affect the complex movements of amyloplasts and the final gravimorphogenesis. In this letter, we implement a diffusive dynamics measurement and inplanta microrheological analysis of amyloplasts in the wild-type plants and actin cytoskeleton mutants for the first time. We found that the intracellular environment of columella cells exhibits the spatial heterogeneity and the cage-confinement on amyloplasts which is the typically characteristics in colloidal suspensions. By comparing the distinct diffusive dynamics of amyloplasts in different types of plants with the behaviors of colloidal systems in different states, we quantitatively characterized the influence of the actin organization dominated intracellular envoronments on the amyloplast movements. Furthermore, the cage-confinement strength was measured by calculating the spatial fluctuation of local apparent viscosity within the columella cells. Finally, a linear association between the initial mechanical stimulation in the columella cells the subsequent intercellular signal transduction and the final gravity response was observed and a possible gravity sensing mechanism was suggested. It suggests the existence of a potential gravity-sensing mechanism that dictates a linear frustration effect of the actin cytoskeleton on the conversion of the mechanical stimulation of amyloplasts into gravitropic signals.
关键词	Plant gravitropism Gravity sensing Amyloplasts Columella cells Microrheology Diffusion Cage-confinement
学科领域	Engineering, Aerospace ; Thermodynamics ; Mechanics
DOI	10.1007/s12217-015-9445-x
收录类别	SCI
语种	英语
WOS关键词	AUXIN ; GRAVITROPISM ; DISRUPTION ; TRANSPORT ; COMPLEX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000366103800010
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1875-0494
资助机构	National Basic Research Program of China [2011CB710902, 2011CB710901] ; NSFC [11104286, 11372314]
作者邮箱	zzy@imech.ac.cn ; zoujunjie@ibcas.ac.cn ; lihanhai@imech.ac.cn ; xueshan1984@yeah.net ; lejie@ibcas.ac.cn ; yurenwang@imech.ac.cn
作品OA属性	Green Published
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25818
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Zhongyu,Zou, Junjie,Li, Hanhai,et al. Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2015,27(6):485-493.
APA	Zheng, Zhongyu,Zou, Junjie,Li, Hanhai,Xue, Shan,Le, Jie,&Wang, Yuren.(2015).Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells.MICROGRAVITY SCIENCE AND TECHNOLOGY,27(6),485-493.
MLA	Zheng, Zhongyu,et al."Dynamical and Microrheological Analysis of Amyloplasts in the Plant Root Gravity-Sensing Cells".MICROGRAVITY SCIENCE AND TECHNOLOGY 27.6(2015):485-493.

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