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| Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification | |
| Lu, Youyuan1; Cheng, Tao2; Zhu, Tiantian; Jiang, Dan1; Zhou, Shiliang2; Jin, Ling; Yuan, Qingjun1; Huang, Luqi1 | |
| 2015 | |
| 发表期刊 | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
 |
| ISSN | 0305-1978 |
| 卷号 | 61页码:488-492 |
| 摘要 | Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | Angelica sinensis Medicinal plant Microsatellite Molecular marker Cross-amplification Population genetics |
| 学科领域 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| DOI | 10.1016/j.bse.2015.07.013 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000361164800068 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 文献子类 | Article |
| 出版地 | OXFORD |
| EISSN | 1873-2925 |
| 资助机构 | National Natural Science Foundation of China (NSFC) [81360615, 81274027, 81130070] ; Beijing Joint Project Specific Funds ; Independent Project of Basic Scientific Research Operating Expenses of Institute of Chinese Materia Medial, China Academy of Chinese Medical Sciences [2011ZDXK-01] |
| 作者邮箱 | 602887952@qq.com ; chengtao@ibcas.ac.cn ; ztt0935@163.com ; jiangxiaodan1027@163.com ; slzhou@ibcas.ac.cn ; zyxyjl@163.com ; yuanqingjun@icmm.ac.cn ; huangluqi01@126.com |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25821 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China 2.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Youyuan,Cheng, Tao,Zhu, Tiantian,et al. Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,61:488-492. |
| APA | Lu, Youyuan.,Cheng, Tao.,Zhu, Tiantian.,Jiang, Dan.,Zhou, Shiliang.,...&Huang, Luqi.(2015).Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,61,488-492. |
| MLA | Lu, Youyuan,et al."Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 61(2015):488-492. |
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| Isolation_and_charac（193KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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