Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology

doi:10.1016/j.talanta.2015.06.069

	Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology
	Zhao, Zhuoya ; Xing, Zenan ; Zhou, Min 1; Chen, Yi 2; Li, Chenzhong 3; Wang, Ruozhong 1; Xu, Wenzhong ; Ma, Mi
	2015
发表期刊	TALANTA
ISSN	0039-9140
卷号	144 页码:502-509
摘要	DELLA proteins and phytohormone gibberellin act together to control convergence point of plant development. A gibberellin-bound nuclear receptor that interacts with the N-terminal domain of DELLA proteins is required for gibberellin induced degradation of DELLA proteins. N-terminal DELLA domain includes two conserved motifs: DELLA and VHYNP. However, their respective functions remain unclear. Meanwhile, the identification and detection of several bioactive gibberellins from the more than 100 gibberellin metabolites are overwhelmingly difficult for their similar structures. Using in vitro biochemical approach, our work demonstrates for the first time that the synthetic GAI N-terminal DELLA domain peptides have similar bioactive function as the expressed protein to interact with AtGID1 a receptor. Furthermore, our results reveal that DELLA motif is vitally important region and DELLA segment is essentially required region to recognize AtGID1a receptor. Finally, based on bioactive GA-dependent of the interaction between AtGID1a and DELLA protein, we generated a new method that could identify and detect bioactive GAs accurately and rapidly with surface plasmon resonance assays. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Bioactive gibberellin DELLA region Gibberellin receptor Qualitative and quantitative analysis SPR Synthetic peptide
学科领域	Chemistry, Analytical
DOI	10.1016/j.talanta.2015.06.069
收录类别	SCI
语种	英语
WOS关键词	ENDOGENOUS GIBBERELLINS ; REGULATES EXPRESSION ; ARABIDOPSIS-THALIANA ; MOLECULAR-MECHANISM ; SIGNALING PATHWAY ; GENE ; IDENTIFICATION ; RECEPTOR ; PLANTS ; RICE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000363346200071
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-3573
资助机构	National Natural Science Foundation of China [90717120, 91117010]
作者邮箱	xuwzh@ibcas.ac.cn
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25827
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth & Dev, Changsha 410128, Hunan, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 4.Florida Int Univ, Dept Biomed Engn, Nanobiosensors Bioelect Lab, Miami, FL 33174 USA
推荐引用方式 GB/T 7714	Zhao, Zhuoya,Xing, Zenan,Zhou, Min,et al. Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology[J]. TALANTA,2015,144:502-509.
APA	Zhao, Zhuoya.,Xing, Zenan.,Zhou, Min.,Chen, Yi.,Li, Chenzhong.,...&Ma, Mi.(2015).Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology.TALANTA,144,502-509.
MLA	Zhao, Zhuoya,et al."Functional analysis of synthetic DELLA domain peptides and bioactive gibberellin assay using surface plasmon resonance technology".TALANTA 144(2015):502-509.

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