Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica

doi:10.1007/s11240-014-0619-9

	Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica
	Li, Yang 1; Shi, Lei1
	2015
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
卷号	120 期号:2 页码:531-538
摘要	To improve storage of green spores by liquid nitrogen (LN), we studied the influence of spore maturity and desiccation process on storage of Osmunda japonica spores. Germination percentages of mature spores after drying for 12 h or equilibrating at 10-55 % relative humidity did not significantly differ from those of fresh spores. So we established that the critical water contents of mature spores were about 6.6 and 4.8 % by silica gel and equilibrating with saturated salt solutions, respectively. Germination percentages of post-mature spores after desication were lower than those of fresh spores. The viability of mature spores was always higher than the viability of post-mature spores following varying periods of storage in LN. The germination percentages of mature spores stored in LN after silica gel desication were comparable to those of mature spores desiccated with saturated salt solution, while desiccation with silica gel led to a marked decrease in germination percentages of post-mature spores during subsequent storage. In general, the level of maturity and desiccation process can have an impact on storage of green spores by LN storage. Mature spores can tolerate desiccation and freezing independent of the desication process. However, slow removal of intracellular water led to less damage and provided more protection to post-mature spores during storage. In addition, reduction in water contents of spores to approx. their critical levels can achieve the best results for long-term storage of spores.
关键词	Osmunda japonica Green spore Maturity level Silica gel desiccation Saturated salt solution desiccation Liquid nitrogen (LN) storage
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-014-0619-9
收录类别	SCI
语种	英语
WOS关键词	EX-SITU CONSERVATION ; FERN SPORES ; FREEZING SENSITIVITY ; MOISTURE-CONTENT ; ZYGOTIC EMBRYOS ; CRYOPRESERVATION ; TEMPERATURE ; VIABILITY ; SEEDS ; WATER
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000348807200010
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5044
资助机构	National Natural Science Foundation of China [31400290] ; National key Technology Support Program of the Ministry of Science and Technology of China [2012BAC01B05] ; Knowledge Innovative Program of The Chinese Academy of Sciences [KSCX2-EW-B-5] ; Special program for technology supporting system of strategic biological resources 'In Vitro Plant Germplasm Collection, BRC-CAS' [CZBZX-1]
作者邮箱	chuanxia97420@163.com
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25837
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Yang,Shi, Lei. Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2015,120(2):531-538.
APA	Li, Yang,&Shi, Lei.(2015).Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica.PLANT CELL TISSUE AND ORGAN CULTURE,120(2),531-538.
MLA	Li, Yang,et al."Effect of maturity level and desiccation process on liquid nitrogen storage of green spores of Osmunda japonica".PLANT CELL TISSUE AND ORGAN CULTURE 120.2(2015):531-538.

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